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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
多聚半乳糖醛酸酶(EC3.2.1.15)是一种细胞壁结合蛋白,可以催化果胶分子中α-(1,4)-聚半乳糖醛酸的裂解,参与果胶的降解,使细胞壁结构解体,导致果实软化,与果实成熟、叶和花的脱落、病原物防御,细胞伸展发育以及木质化有关,在植物抗病性和食品贮藏保鲜领域具有较高的研究价值。 测定原理:多聚半乳糖醛酸酶水解果胶酸生成半乳糖醛酸,具有还原性醛基,与DNS试剂反应生成红棕色物质,在540nm有特征吸收峰,测定540nm处吸光值变化可计算得多聚半乳糖醛酸酶活性。
Polygalacturonase (EC 3.2.1.15) is a cell wall-bound protein that catalyzes the cleavage of α-(1,4)-polygalacturonic acid in pectin molecules, participating in pectin degradation and leading to the disintegration of cell wall structure, which results in fruit softening. It is associated with fruit ripening, leaf and flower abscission, pathogen defense, cell expansion and development, as well as lignification, and holds significant research value in the fields of plant disease resistance and food storage and preservation. Determination Principle: Polygalacturonase hydrolyzes pectic acid to produce galacturonic acid, which contains a reducing aldehyde group. This reacts with the DNS reagent to form a reddish-brown compound with a characteristic absorption peak at 540 nm. Polygalacturonase activity can be calculated by measuring the change in absorbance at 540 nm.