Name: 多糖多酚植物基因组DNA提取试剂盒
SKU: P1522831
Availability: InStock

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为什么选择此级别

BioReagent,for DNA and RNA applications,分子生物学级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

本试剂盒采用特异性结合DNA的吸附柱和优化的缓冲体系，能从多种植物中分离纯化高质量基因组DNA，优化的溶液可以有效去除多糖多酚植物样本中的蛋白质、多糖以及酚类等杂质。本试剂盒提取的基因组DNA产量大、纯度高，适用于PCR、荧光定量PCR、分子标记和文库构建等实验。

产品号	组件	物理外观	50T	储存温度
P1522831A	Buffer PPL	液体	33 mL	RT.
P1522831B	Buffer PPT	液体	12 mL	RT.
P1522831C	Buffer PA	液体	33 mL	RT.
P1522831D	Buffer RW1	液体	15 mL	RT.
P1522831E	Buffer EB	液体	15 mL	RT.
P1522831F	RNase A (10 mg/mL)	液体	550 μL	RT.
P1522831G	过滤柱GS (含2mL收集管)	—	1 EA×50	RT.
P1522831H	吸附柱WHF (含2mL收集管)	—	1 EA×50	RT.

本试剂盒所有组分置于室温干燥条件下，可保存15个月。

操作步骤

使用前请先在Buffer RW1中加入23 mL的无水乙醇。

1. 样本处理（两种研磨方式二选一）

1.1 组织研磨仪破碎法

在2 mL研磨管中加入1颗钢珠，然后加入50-100 mg新鲜植物材料或15-20 mg干燥植物材料；液氮预冷后，以40 Hz、30 sec的条件充分研磨成粉末。向研磨管中加入600 µL Buffer PPL和10 µL RNase A，迅速涡旋振荡混匀；将离心管置于65℃金属浴或水浴中孵育15 min，孵育过程中颠倒离心管数次，使样品充分混合。

1.2 液氮研磨法

称量经液氮充分研磨好的新鲜植物材料粉末约50-100 mg，或干燥植物材料粉末约15-20 mg；迅速将其转移到预先装有600 µL Buffer PPL的1.5 mL离心管中，加入10 µL RNase A，迅速涡旋振荡混匀；将离心管置于65℃金属浴或水浴中孵育15 min，孵育过程中颠倒离心管数次，使样品充分混合。

2. 向步骤1处理后的样品中加入200 µL Buffer PPT，涡旋振荡混匀1 min；12,000 rpm (13,680×g) 离心5 min，吸取全部上清液至过滤柱GS（将过滤柱放入配套的2 mL收集管中）；12,000 rpm (13,680 × g) 离心1 min，将收集管中的滤液转移至新的1.5 mL离心管中，弃去过滤柱和废液。

3. 向步骤2得到的滤液中加入600 µL Buffer PA，充分混合均匀，此时可能会出现絮状沉淀（属于正常现象）；将所得溶液和沉淀全部加入到吸附柱WHF（将吸附柱放入配套的2 mL收集管中），每次加入量不超过700 μL，若体积超出可分两次转入；12,000 rpm (13,680 × g) 离心30 sec，弃去收集管中的废液，将吸附柱重新放回收集管中。

4. 向吸附柱中加入700 µL Buffer RW1（使用前已加入无水乙醇），12,000 rpm (13,680×g) 离心30 sec，弃去废液，将吸附柱放回收集管中。

5. 向吸附柱中加入700µL 75%乙醇（客户自备），12,000 rpm (13,680×g) 离心30 sec，弃去废液，将吸附柱放回收集管中。

6. 重复步骤5（第二次洗涤）的操作，进一步去除吸附柱上残留的多糖、多酚、盐分等杂质，提升DNA纯度。

7. 将吸附柱连同收集管一起，12,000 rpm (13,680 × g) 离心2 min，彻底去除吸附柱中残留的洗涤液和杂质（避免残留乙醇影响后续DNA洗脱和实验）。

8. 将吸附柱从收集管中取出，转入一个新的1.5 mL离心管中，开盖放置2 min，彻底挥发吸附柱中残留的乙醇。向吸附膜的中间部位缓慢悬空滴加50-100 μL Buffer EB，室温放置1 min；12,000 rpm (13,680×g) 离心30 sec，收集离心管中的DNA溶液，可立即用于后续实验，或置于-20℃长期保存。

【注意】

(1) 如果下游实验对pH值或EDTA敏感，可以用灭菌水洗脱。洗脱液的pH值对洗脱效率有很大影响，若用水做洗脱液应保证其pH值在7.0-8.5（可以用NaOH将水的pH值调到此范围），pH值低于7.0时洗脱效率不高。

(2) 如果要提高DNA的终浓度，可以将步骤8所得的DNA洗脱液重新加至吸附膜上，室温放置2 min，12,000 rpm (13,680×g) 离心30 sec。如果所得DNA的量小于1 μg，建议使用50 μL Buffer EB进行洗脱。

(3) 如需长期保存，建议使用Buffer EB洗脱并于-20℃长期保存，可有效防止DNA降解。

DNA浓度及纯度检测

提取的基因组DNA片段大小，与样品保存时间、操作过程中的剪切力等因素有关。纯化得到的DNA片段可用琼脂糖凝胶电泳和紫外分光光度计两种方法，检测其浓度与纯度。

1. 浓度检测

通过紫外分光光度计测量样品在260 nm处的吸光度（A₂₆₀），确定DNA浓度。若需稀释样品，建议使用Buffer EB进行稀释，用水稀释样本可能导致数值不准确。260 nm处准确的吸光度应在0.1-1.0范围内，以保证检测结果的准确性。换算标准：260 nm处1个单位的吸光度，相当于50µg/mL双链DNA，或40µg/mL单链DNA。

2. 纯度检测

通过检测A₂₆₀/A₂₈₀的比值判断DNA纯度，合格的基因组DNA，其A₂₆₀/A₂₈₀比值应在1.7-1.9之间。如果洗脱时未使用Buffer EB，而是使用ddH₂O，比值会偏低，因为pH值和离子存在会影响光吸收值，但并不表示DNA纯度低。

This kit uses a DNA‑specific binding spin column and an optimized buffer system to isolate and purify high‑quality genomic DNA from a variety of plants. The optimized solutions effectively remove proteins, polysaccharides, phenols, and other impurities from polysaccharide‑ and polyphenol‑rich plant samples. The genomic DNA extracted with this kit has high yield and high purity, and is suitable for applications such as PCR, real‑time PCR, molecular markers, and library construction.

Product No.	Component	Appearance	50T	Storage
P1522831A	Buffer PPL	Liquid	33 mL	RT
P1522831B	Buffer PPT	Liquid	12 mL	RT
P1522831C	Buffer PA	Liquid	33 mL	RT
P1522831D	Buffer RW1	Liquid	15 mL	RT
P1522831E	Buffer EB	Liquid	15 mL	RT
P1522831F	RNase A (10 mg/mL)	Liquid	550 μL	RT
P1522831G	GS Filter Column (with 2 mL collection tube)	—	1 EA × 50	RT
P1522831H	WHF Spin Column (with 2 mL collection tube)	—	1 EA × 50	RT

All components of this kit can be stored under dry conditions at room temperature for 15 months.

Procedure

Before use, add 23 mL of absolute ethanol to Buffer RW1.

1. Sample Processing (Choose one of two grinding methods)

1.1 Tissue grinder disruption method

Add one steel bead to a 2 mL grinding tube, then add 50–100 mg of fresh plant material or 15–20 mg of dried plant material. Pre‑cool with liquid nitrogen, then grind thoroughly at 40 Hz for 30 sec. Add 600 µL of Buffer PPL and 10 µL of RNase A, and vortex immediately to mix. Incubate the tube in a 65°C metal bath or water bath for 15 min. During incubation, invert the tube several times to mix the sample thoroughly.

1.2 Liquid nitrogen grinding method

Weigh about 50–100 mg of fresh plant material powder or 15–20 mg of dried plant material powder that has been thoroughly ground in liquid nitrogen. Quickly transfer the powder into a 1.5 mL centrifuge tube pre‑filled with 600 µL of Buffer PPL. Add 10 µL of RNase A and vortex immediately to mix. Incubate the tube in a 65°C metal bath or water bath for 15 min. During incubation, invert the tube several times to mix the sample thoroughly.

2. Add 200 µL of Buffer PPT to the sample processed in step 1. Vortex for 1 min to mix. Centrifuge at 12,000 rpm (13,680 × g) for 5 min. Transfer all the supernatant to a GS Filter Column (placed in the provided 2 mL collection tube). Centrifuge at 12,000 rpm (13,680 × g) for 1 min. Transfer the filtrate from the collection tube to a new 1.5 mL centrifuge tube. Discard the filter column and the waste.

3. Add 600 µL of Buffer PA to the filtrate obtained in step 2 and mix thoroughly. Flocculent precipitate may appear (this is normal). Transfer the entire solution and precipitate into a WHF spin column (placed in the provided 2 mL collection tube). Add no more than 700 μL per transfer; if the volume exceeds, transfer in two portions. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Discard the flow‑through and return the spin column to the collection tube.

4. Add 700 µL of Buffer RW1 (with ethanol pre‑added) to the spin column. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Discard the flow‑through and return the spin column to the collection tube.

5. Add 700 µL of 75% ethanol (user‑supplied) to the spin column. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Discard the flow‑through and return the spin column to the collection tube.

6. Repeat step 5 (second wash) to further remove residual polysaccharides, polyphenols, salts, and other impurities on the spin column, thereby increasing DNA purity.

7. Centrifuge the spin column together with the collection tube at 12,000 rpm (13,680 × g) for 2 min to completely remove residual wash buffer and impurities (this avoids residual ethanol affecting subsequent DNA elution and experiments).

8. DNA elution

Transfer the spin column to a new 1.5 mL centrifuge tube. Open the cap and let stand for 2 min to allow residual ethanol to evaporate completely. Slowly add 50–100 μL of Buffer EB dropwise to the center of the membrane without touching it. Let stand at room temperature for 1 min. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Collect the DNA solution in the centrifuge tube. The DNA can be used immediately for downstream experiments or stored at –20°C for long‑term preservation.

【Notes】

(1) If downstream applications are sensitive to pH or EDTA, sterile water can be used for elution. The pH of the elution buffer significantly affects elution efficiency. If water is used, ensure its pH is between 7.0 and 8.5 (adjust with NaOH). pH values below 7.0 will reduce elution efficiency.

(2) To increase the final DNA concentration, reload the collected DNA eluate from step 8 onto the same membrane, let stand at room temperature for 2 min, centrifuge at 12,000 rpm (13,680 × g) for 30 sec. If the amount of DNA obtained is less than 1 μg, it is recommended to elute with 50 μL of Buffer EB.

(3) For long‑term storage, it is recommended to elute with Buffer EB and store at –20°C to effectively prevent DNA degradation.

DNA Concentration and Purity Assessment

The fragment size of the extracted genomic DNA is influenced by factors such as sample storage time and shear forces during handling. The purified DNA can be assessed for concentration and purity by agarose gel electrophoresis and UV spectrophotometry.

1. Concentration determination

Measure the absorbance of the sample at 260 nm (A₂₆₀) using a UV spectrophotometer to determine DNA concentration. If dilution is required, Buffer EB is recommended. Dilution with water may lead to inaccurate readings. The absorbance at 260 nm should be kept within 0.1–1.0 to ensure accuracy. Conversion: 1 unit of absorbance at 260 nm corresponds to 50 µg/mL of double‑stranded DNA or 40 µg/mL of single‑stranded DNA.

2. Purity assessment

Evaluate DNA purity by the A₂₆₀/A₂₈₀ ratio. For high‑quality genomic DNA, the A₂₆₀/A₂₈₀ ratio should be between 1.7 and 1.9. If ddH₂O instead of Buffer EB is used for elution, the ratio may be lower due to the influence of pH and ions on light absorption; this does not necessarily indicate low DNA purity.

规格

别名

多糖多酚植物基因组DNA抽提试剂盒(离心柱式)

英文别名

Plant Plus DNA Kit

规格或纯度

BioReagent, 分子生物学级, for DNA and RNA applications

英文名称

Polysaccharide & Polyphenol Rich Plants DNA Kit

应用

DNA Extraction, 核酸提取与纯化

储存与运输

储存条件

室温

运输条件

常规运输

稳定性与储存

Store at room temperature long term (15 months).

组分与储存

产品号	组件	物理外观	50T	储存温度
P1522831A	Buffer PPL	Liquid	33 mL	RT
P1522831B	Buffer PPT	Liquid	12 mL	RT
P1522831C	Buffer PA	Liquid	33 mL	RT
P1522831D	Buffer RW1	Liquid	15 mL	RT
P1522831E	Buffer EB	Liquid	15 mL	RT
P1522831F	RNase A (10 mg/mL)	Liquid	550 μL	RT
P1522831G	过滤柱GS (含2mL收集管)	—	1 EA × 50	RT
P1522831H	吸附柱WHF (含2mL收集管)	—	1 EA × 50	RT

技术文档

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高级数据

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批号(Lot Number)	证书类型	货号
ZJ26F0636885	分析证书	P1522831

技术文档和文章

p1522831 多糖多酚植物基因组dna提取试剂盒说明书.pdf

p1522831 polysaccharide polyphenol rich plants dna kit manual.pdf

DNA提取方法比较：酚氯仿法、硅胶柱法、磁珠法、CTAB法与快速裂解法

目录：技术文章

探索主题:

DNA提取磁珠法基因组DNA 酚氯仿抽提硅胶柱法 CTAB法 DNA纯化核酸质量评价

Comparison of DNA Extraction Methods: Phenol-Chloroform Extraction, Silica Column Method, Magnetic Bead Method, CTAB Method, and Rapid Lysis Method

目录：技术文章

探索主题:

DNA extraction Genomic DNA Phenol-chloroform extraction Silica column method Magnetic bead method CTAB method DNA purification Nucleic acid quality assessment

溶液计算器

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计算溶液所需的质量、体积或浓度。

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浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

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多糖多酚植物基因组DNA提取试剂盒

储存与运输

质量文档

文献证明

概述

规格

技术文档

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✅ 分析证书 (COA)

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