在组织切片过程中一些组织内含有骨质或钙化灶时，含钙的组织不宜直接用石蜡包埋切片，这是因为钙和石蜡之间的密度不同，较难切出完整的切片。对含钙组织最好固定之后，再进行脱钙或二者同时进行，在脱钙过程中酸类物质打破了组织中蛋白质分子间的横链，并释放可以同水分子结合的亲水基团，引起组织膨胀；组织脱钙后要立即将组织中的酸中和掉，使组织迅速脱酸和中性化，以保证切片质量和染色效果。
本品主要由硫酸钠或硫酸锂、去离子水等组成，组织经酸性脱钙液后需经本产品进行过夜处理。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
1、骨组织脱钙时，取材不易过厚，一般大约 5mm。
2、组织经甲酸、盐酸、硝酸等脱钙后用蒸馏水清洗 3 次，每次 20min。
3、组织转移至 20~30 倍体积的脱钙后碱处理液中，经过夜处理，建议 16 小时以上。
4、用流水冲洗 2~3 小时。进行后续实验。
注意事项:
1、适当加温能加快处理速度，一般不应超过 37~40℃。
2、不建议采用水洗处理法，易引起组织膨胀。
附：乙醇处理法：70% 的乙醇处理，每 1h 更换一次，重复 2~3 次。
When tissues contain bone tissue or calcified lesions during tissue sectioning, calcium-containing tissues cannot be directly embedded in paraffin for sectioning. This is due to the density difference between calcium and paraffin, which makes it difficult to obtain intact sections.
For calcium-containing tissues, decalcification is preferably carried out after fixation, or fixation and decalcification can be performed simultaneously. During decalcification, acidic substances break the cross-links between protein molecules in tissues and release hydrophilic groups that bind to water molecules, resulting in tissue swelling.Immediately after decalcification, the residual acid in the tissue must be neutralized to rapidly deacidify and neutralize the tissue, so as to guarantee sectioning quality and staining results.
This product is mainly composed of sodium sulfate or lithium sulfate, deionized water and other ingredients. Tissues treated with acidic decalcifying solution shall be soaked in this product overnight.This reagent is only intended for scientific research and shall not be used for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. When sampling bone tissue for decalcification, the tissue block should not be overly thick, with a recommended thickness of approximately 5 mm.
2. After decalcification with formic acid, hydrochloric acid, nitric acid or other acids, rinse the tissue with distilled water 3 times for 20 minutes each time.
3. Transfer the tissue into Post-Decalcification Alkaline Treatment Solution with a volume 20 to 30 times that of the tissue sample, and perform overnight treatment for no less than 16 hours as recommended.
4. Rinse the sample with running tap water for 2 to 3 hours before proceeding with subsequent experiments.
Precautions
1. Appropriate heating can accelerate the treatment process, and the temperature should not exceed 37~40℃.
2. Rinsing with pure water is not recommended, as it tends to cause tissue swelling.
Appendix: Ethanol Treatment Method: Treat the sample with 70% ethanol, replace the ethanol every 1 hour, and repeat the operation 2 to 3 times.
ZJ26F0939298
危险品化学品经营许可证（带存储）