计算溶液所需的质量、体积或浓度。
Plank-Rychlo脱钙液
有货
为什么选择此级别
BioReagent,即用型 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
在组织切片过程中，一些组织内含有骨质或钙化灶时，含钙的组织不宜直接用石蜡包埋切片，这是因为钙和石蜡之间的密度不同，较难切出完整的切片；对含钙组织最好固定之后，再进行脱钙或二者同时进行，然后进行下游操作如脱水、透明、浸蜡、包埋、切片，用于脱钙的试剂很多，脱钙剂包括有机酸、无机酸、乙二胺四乙酸 (EDTA) 以及电解法脱钙。
Plank‑Rychlo脱钙液主要由稀酸、氯化铵或氯化铝、甲酸等组成，其优点是：
①作用迅速，比 JYBL‑Ⅰ (阿拉丁货号：J1525466)时间更短，一般 24 h 即可；
②对组织的结构损害小；
③脱钙完全；该脱钙液特别适用于日常病理科骨组织标本脱钙，但不宜用化学方法确定脱钙终点，对组织结构有一定损伤。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料:
1、蒸馏水或 PBS
2、加热装置或微波炉、脱钙专用容器 (玻璃)
操作步骤 (仅供参考):
1、骨组织脱钙时，取材不易过厚，一般大约 3~5 mm。
2、组织固定后，用蒸馏水或 PBS 清洗 3 次，每次 20 min。
3、组织转移至 20~30 倍体积的 Plank‑Rychlo 脱钙液中，脱钙 24 h；如果想加快脱钙速度，可以置于 37℃进行脱钙。
4、用蒸馏水冲洗数次。
5、常规脱水、包埋。
注意事项:
1、骨组织脱钙前应充分固定，组织块尽量小。
2、骨组织脱钙应先固定后脱钙或脱钙固定同时进行，不应先脱钙后固定，以便减少组织的损伤程度。
3、厚度 5 mm 的骨组织块脱钙时间一般脱钙 24~36 h 即可。
4、适当加温能加快脱钙的速度，一般不应超过 37~40℃，温度过高容易使骨组织造成松散解体，尤其不可大于 60℃。
5、每隔一段时间检测一次脱钙程度。脱钙应彻底，防止脱钙不足或过度，脱钙过度会增加组织的损伤程度，影响染色结果。脱钙程度应控制在不影响切片的同时尽量缩短时间。
6、脱钙用具避免使用金属容器。
7、脱钙完成后用自来水彻底冲洗标本，以免影响染色。
8、为了您的安全和健康，请穿实验服并戴一次性手套操作。
附录:
脱钙终点的测定 (物理法): 采用针刺、手掐、钳夹等方法，当骨组织变软或针刺时没有阻力感即可终止脱钙；物理检测法会对组织结构有一定的损害，尽量避免用力过大或反复检测。
During tissue sectioning, tissues containing bone or calcified lesions cannot be directly embedded in paraffin for sectioning. Due to the density difference between calcium and paraffin, intact sections are difficult to obtain. For calcium‑containing tissues, decalcification should be performed after fixation, or fixation and decalcification can be carried out simultaneously, followed by downstream procedures including dehydration, clearing, wax infiltration, embedding and sectioning. A wide range of decalcifying agents are available, such as organic acids, inorganic acids, ethylenediaminetetraacetic acid (EDTA), and electrolytic decalcification.
Plank‑Rychlo Decalcifying Solution is mainly composed of dilute acid, ammonium chloride or aluminium chloride, formic acid and other components. Its advantages are as follows:
① Fast‑acting, with shorter processing time than JYBL‑Ⅰ (Aladdin Cat.No.: J1525466), generally complete within 24 h;
② Low damage to tissue structure;
③ Thorough decalcification.
This decalcifying solution is especially suitable for routine decalcification of bone specimens in pathology laboratories. However, chemical methods are not recommended for determining the decalcification endpoint, and it may cause certain damage to tissue architecture.
This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Materials to be prepared
1. Distilled water or PBS
2. Heating device or microwave oven, dedicated glass container for decalcification
Operating Procedures (For Reference Only)
1. For bone‑tissue decalcification, samples should not be excessively thick; the recommended thickness is approximately 3‑5 mm.
2. After tissue fixation, rinse samples 3 times with distilled water or PBS, 20 min each time.
3. Transfer tissues into Plank‑Rychlo Decalcifying Solution at 20‑30‑fold volume and decalcify for 24 h. To accelerate decalcification, incubate at 37 ℃.
4. Rinse several times with distilled water.
5. Proceed with standard dehydration and embedding.
Precautions
1. Bone tissues shall be fully fixed prior to decalcification, and tissue blocks should be kept as small as possible.
2. Bone tissues should be decalcified after fixation, or fixation and decalcification shall be performed concurrently. Decalcification prior to fixation is not allowed to minimize tissue damage.
3. For 5 mm‑thick bone blocks, decalcification usually takes 24‑36 h.
4. Moderate heating accelerates decalcification; temperature should generally not exceed 37‑40 ℃. Excessively high temperature may cause bone tissue disintegration, and the temperature must never exceed 60 ℃.
5. Check the decalcification progress periodically. Complete decalcification is required to avoid under‑decalcification or over‑decalcification. Over‑decalcification aggravates tissue damage and compromises staining results. Decalcification should be sufficient for sectioning while keeping the processing time as short as possible.
6. Avoid metal containers for decalcification procedures.
7. Thoroughly rinse specimens with tap water upon completion of decalcification to prevent interference with staining.
8. Wear a lab coat and disposable gloves for your safety and health during operation.
Appendix
Determination of Decalcification Endpoint (Physical Method):
Use needle piercing, pinching or forceps clamping. Terminate decalcification when bone tissue softens or no resistance is felt during needle piercing. Physical detection may cause certain damage to tissue structure; avoid excessive force and repeated testing.
规格
规格或纯度
BioReagent, 即用型
稳定性与储存
Store at RT long term (12 months).
英文名称
Plank-Rychlo Decalcifying Solution
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
产品属性
| pH | 7.4 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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