计算溶液所需的质量、体积或浓度。
BioReagent,for western blot,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丽春红是一种常用蛋白质染色剂。试剂本身带负电荷,既可与带正电荷的氨基酸残基相结合,也可与蛋白质非极性区结合,从而在印迹膜上形成清晰的红色条带;同时这种结合具有可逆性,染色后可用纯水、PBS缓冲液等进行漂洗脱色,对后续实验不产生影响。本产品可用于检测Western Blot实验中PVDF或NC膜上的蛋白,以检测蛋白的转膜效率,具有灵敏度高,使用方便等优点,对蛋白的检测下限为250ng。
使用说明
1、蛋白转移完毕后,将PVDF或NC膜完全浸没在 丽春红染液中,摇动染色3~5min;
2、使用纯水、PBS 缓冲液或其它适当溶液对印迹膜进行漂洗2~3次,每次3~5min,去除未与蛋白结合的染料,直至膜上出现清晰条带;
3、检测结束后,再次使用纯水,PBS缓冲液或其它合适的溶液漂洗印迹膜2~3次,每次3~5min,去除与蛋白结合的丽春红染料;
4、按照正常步骤,进行后续的Western Blot检测。
注意事项
1、丽春红染液不适用于尼龙膜上蛋白的检测;
2、为了您的安全和健康,请穿实验服并戴一次性手套操作;
3、本产品仅限科研使用。
Ponceau S is a commonly used protein stain. As a negatively charged reagent, it binds to both positively charged amino acid residues and non-polar regions of proteins, producing distinct red bands on blotting membranes. This binding is reversible, allowing the stain to be washed off with pure water or PBS buffer after use without interfering with subsequent experiments. This product is designed for detecting proteins on PVDF or nitrocellulose (NC) membranes in Western Blot experiments to assess transfer efficiency. It offers high sensitivity and ease of use, with a detection limit of 250 ng for proteins.
After protein transfer, fully immerse the PVDF or NC membrane in Ponceau S staining solution. Agitate on a shaker for 3–5 minutes.
Rinse the membrane 2–3 times with pure water, PBS buffer, or another appropriate solution (3–5 minutes per rinse) to remove unbound dye until clear protein bands appear.
After detection, rinse the membrane again 2–3 times with pure water, PBS buffer, or another suitable solution (3–5 minutes per rinse) to remove Ponceau S bound to proteins.
Proceed with standard Western Blot detection steps.
Ponceau S staining solution is not suitable for nylon membranes.
Wear a lab coat and disposable gloves for safety.
For research use only.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P155952 | |
| 分析证书 | P155952 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 for Western blot 级别指南 →