计算溶液所需的质量、体积或浓度。
无机焦磷酸酶(PPase)普遍存在于自然界,在能量代谢中具有重要作用,可以为蛋白、RNA和DNA的生物合成反应提供热力学动力。克隆并测序了编码无机焦磷酸酶(PPase)的大肠杆菌K-12基因ppa,通过引物延伸图谱已鉴定ppa mRNA的5′端。
来源:携带E.coli K-12 焦磷酸酶质粒的E.coli 菌株。
应用:
• 蛋白质、RNA和DNA的合成
• 催化 PPi + H₂O → 2Pi 的反应
Inorganic pyrophosphatase (PPase) is ubiquitous in nature and plays an important role in energy metabolism, provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Escherichia coli K-12 gene ppa encoding inorganic pyrophosphatase (PPase) was cloned and sequenced. The 5’-end of the ppa mRNA was identified by primer extension mapping.
Source: E. coli strain carrying a plasmid encoding pyrophosphatase from E. coli K-12.
Application:
- Role in protein, RNA, and DNA synthesis
- Catalyzing the reaction PPi + H₂O → 2Pi
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