计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,干燥。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the polar lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells .
In Vitro
General Protocol (Example for Py-BODIPY NHS ester) 1.Preparation of Py-BODIPY NHS ester working solution 1.1Preparation of the stock solution Dissolve 1 mg Py-BODIPY NHS ester in 382 μL DMSO to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20 ℃ -80 ℃ away from light and avoid repetitive freeze-thaw cycles. 1.2Preparation of BODIPY 493/503 working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution. Note: Please adjust the concentration of Py-BODIPY NHS ester working solution according to the actual situation. 2.Cell staining 2.1 Suspension cells (6-well plate) a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 mL. b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes. c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d.Wash twice with PBS, 5 minutes each time. e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
规格或纯度
≥98%
英文名称
Py-BODIPY-NHS ester
生化机理
BODIPY 染料是一种小分子染料，具有很强的紫外吸收能力，其荧光峰比较尖锐，量子产率高。它们对环境的极性和酸碱度相对不敏感，且相对稳定。
储存条件
2-8°C储存,避光,干燥
运输条件
低温运输
纯度
≥98%
产品线
生物产品
名称和识别符
分子类型
小分子
分子量
426.18
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 25 mg/mL (58.66 mM; Need ultrasonic)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C、避光、干燥条件下保存。请与干燥剂一同密封保存，本品对湿气敏感。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 201998-61-0，分子式为 C20H17BF2N4O4，分子量为 426.18 g/mol。
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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