计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
PY-60 is a robust and specific activator of YAP transcriptional activity that targets annexin A2 (ANXA2) with a K d of 1.4 µM. PY-60 directly binds to ANXA2 and antagonizes its normal cellular function of repressing YAP activity.
In Vitro
PY-60 targets ANXA2 to activate YAP. ?\nPY-60, a thiazole-substituted derivative, dose-dependently induces luciferase activity in 293A-TEAD-LUC cells in the presence or absence of serum when cells were plated at high cell density (EC 50 =?1.5 and 1.6?μM, respectively). PY-60 treatment also dose-dependently promoted the association of YAP and TEAD proteins in cells and induced the nuclear localization of YAP in response to increased cell density. PY-60 robustly increases the levels of YAP-controlled transcripts (that is, ANRKD1 , CYR61 and CTGF ) in 293A cells and other human cell lines (that is, MCF10A, HEK293T, H69 and HaCaT), but did not augment the levels of YAP itself ( YAP1 ). ?\nPY-60 activates a proproliferative, YAP-dependent transcriptional program in the adult animal capable of remodeling the epidermis through proliferation. ?\nPY-60 liberates the ANXA2-YAP complex from the cell membrane and competes for ANXA2 binding of phosphoinositides. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
PY-60 (10?uM; applied topically to the dorsal skin of wild-type adult C57BL/6 mice over the course of 10?days) promotes a dramatic expansion of keratinocytes and K14-positive precursors, as assessed by hematoxylin and eosin and anti-K14 histological staining at the study end. PY-60 results in an approximate doubling of epidermal thickness, a result derived from an increased number of keratinocytes per unit length of skin . MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
规格或纯度
≥98%
英文名称
PY-60
生化机理
PY-60是一种强效、特异性的YAP转录活性激活剂，以附件素A2（ANXA2）为靶标，K d为1.4 µM。PY-60可直接与ANXA2结合，并拮抗其抑制YAP活性的正常细胞功能。
储存条件
-20°C储存
运输条件
超低温运输
作用类型
激活剂
纯度
≥98%
名称和识别符
分子类型
小分子
Isomeric SMILES
C1=CC=C(C=C1)C2=CC(=NO2)C(=O)NCCCC3=NC=CS3
PubChem CID
分子量
313.37
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 100 mg/mL (319.11 mM; Need ultrasonic)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 2765218-56-0，分子式为 C16H15N3O2S，分子量为 313.37 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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