计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
PTUPB is a potent and dual sEH and COX-2 enzymes inhibitor with IC 50 of 0.9 nM and 1.26 μM, respectively.
In Vitro
PTUPB (1-10 μM; 24 hours) shows an inhibitory activity against human 5-LOX, exhibits a 83% and 44% inhibition at 10 μM and 1 μM, respectively. PTUPB (10-20 μM; 72 hours) has minimal inhibitory effects on cell proliferation in multiple cancer cell lines, including human melanoma cell and a transformed endothelial cell, whereas it potently inhibits HUVEC proliferation after 3 days of application. PTUPB (10-20 μM; 72 hours) induces cell cycle arrest at the G0/1 phase at different various. The percentage of cell number of PTUPB are 65.15%, 66.87%,and 65.91% at 10 μM, 15 μM, and 20 μM, respectively. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Multiple cancer cell lines: PC-3 cells, Met-1, H-1, A375, and transformed endothelial cell line (bEnd.3) Concentration: 10 μM, 15 μM, and 20 μM Incubation Time: 72 hours Result: Inhibited HUVEC proliferation after 3 days. Cell Cycle AnalysisCell Line: HUVECs Concentration: 10 μM, 15 μM, and 20 μM Incubation Time: 72 hours Result: Induced cell cycle arrest at the G0/1 phase.
In Vivo
PTUPB (subcutaneous injection; 30 mg/kg; 4 weeks) inhibits LLC tumor growth by 70-83% and exhibits with no overt toxicity, such as any weight loss when it is compared with the control group. After a period of treatment, the peak plasma concentration of PTUPB is high . PTUPB (subcutaneous injection; 5 mg/kg; once daily; 12 weeks) ameliorates high-fat diet-induced non-alcoholic fatty liver disease via inhibiting NLRP3 inflammasome activation. It reduces body weight, liver weight, liver triglyceride and cholesterol content. It also decreases the expression of lipolytic/lipogenic and lipid uptake related genes. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: C57BL/6 mice with LLC cells Dosage: 30 mg/kg; 4 weeks Administration: Subcutaneous injection via Alzet osmotic minipumps; once daily; 4 weeks Result: Inhibited LLC tumor growth and metastasis. Animal Model: High-fat diet (HFD)-induced obeseadult male C57BL/6 miceDosage: 5 mg/kg; 12 weeks Administration: Subcutaneous injection; once daily; 12 weeks Result: Arrested fibrotic progression and ameliorated high-fat diet-induced non-alcoholic fatty liver disease.
规格
规格或纯度
10mM in DMSO
英文名称
PTUPB
生化机理
PTUPB 是一种强效的 sEH 和 COX-2 双酶抑制剂，其 IC 50 分别为 0.9 nM 和 1.26 μM。
储存条件
-80℃储存
运输条件
特低温运输
名称和识别符
分子类型
小分子
Isomeric SMILES
C1=CC=C(C=C1)C2=CC(=NN2C3=CC=C(C=C3)S(=O)(=O)N)CCCNC(=O)NC4=CC=C(C=C4)C(F)(F)F
MeSH Entry Terms
1-(3-(5-phenyl-1-(4-sulfamoylphenyl)pyrazol-3-yl)propyl)-3-(4-(trifluoromethyl)phenyl)urea;dual inhibitor PTUPB
分子量
543.56
技术文档
高级数据
关联靶点（人）
EPHX2
Tchem
双功能环氧化物水解酶2(Bifunctional epoxide hydrolase 2)
(1 活性数据)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于?80 °C条件下保存。需超低温保存以维持规定的有效期。
本产品如何运输？
本产品采用干冰配冷袋冷冻运输。收货后请立即拆包，并按上述储存条件保存；处理干冰时请戴隔热手套并保持通风。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 1287761-01-6，分子式为 C26H24F3N5O3S，分子量为 543.6 g/mol。
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