计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
物理外观: Liquid
产品介绍:
在细胞或组织提取过程中,靶蛋白可能会被细胞中天然存在的蛋白酶分解,从而影响后续的蛋白质检测。本产品为经优化和测试的用于常规细胞或组织蛋白提取等的蛋白酶抑制剂混合物,含有6种广谱蛋白酶抑制剂(104mM AEBSF, 80μM Aprotinin, 5mM Bestatin, 1.5mM E-64, 2mM Leupeptin and 1.5mM Pepstatin A in DMSO),保护蛋白完整性。
使用方法:
在室温下溶解DMSO储液,在您的实验体系中,按照1: 100 的比率(体积比体积)将抑制剂混合物加入到样品溶液中(比如细胞裂解液或者组织抽提物)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
注意事项:
避免反复冻融,第--次使用后,可按照每次使用量分装冻存,并应在临使用前添加到裂解液等适当溶液中。
本产品中含有的蛋白酶抑制剂对人体有害,为了您的安全和健康,请穿实验服并戴一-次性 手套操作。
During the extraction of proteins from cells or tissues, target proteins may be degraded by endogenous proteases, which interferes with subsequent protein detection. This product is an optimized and validated protease inhibitor cocktail for routine protein extraction from cells and tissues. It contains six broad-spectrum protease inhibitors (104 mM AEBSF, 80 μM Aprotinin, 5 mM Bestatin, 1.5 mM E-64, 2 mM Leupeptin and 1.5 mM Pepstatin A dissolved in DMSO) to preserve protein integrity.
Instructions for Use:
Thaw the DMSO stock solution at room temperature. Add the inhibitor cocktail to your sample solution (such as cell lysate or tissue extract) at a volume ratio of 1:100. Lysates supplemented with the protease inhibitor cocktail should be freshly prepared before use and shall not be frozen for later storage.
Precautions:
1. Avoid repeated freeze-thaw cycles. After initial use, aliquot the stock solution according to single-use volumes and freeze for storage. Add the cocktail to lysate or other appropriate solutions right before experiments.
2. The protease inhibitors contained in this product are hazardous to human health. Please wear a lab coat and disposable gloves during operation for your personal safety.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P665818 | |
| 分析证书 | P665818 | |
| 分析证书 | P665818 | |
| 分析证书 | P665818 | |
| 分析证书 | P665818 | |
| 分析证书 | P665818 |