计算溶液所需的质量、体积或浓度。
BioReagent,无菌,无菌过滤,适用于免疫组织化学(for IHC) 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
物理外观:Liquid
产品介绍:
冰冻切片快速抗原修复液(5×)含有SDS等抗原修复试剂等,pH值约为7.4,可以快速有效地去除醛类固定试剂导致的蛋白之间的交联,充分暴露石蜡切片等样品中的抗原表位,从而大大改善免疫染色效果。它特别适合用于冰冻切片的快速抗原修复,只需室温孵育5min即可完成对冰冻切片的抗原修复。
操作流程:
1、试剂准备
1.1 冰冻切片快速抗原修复液(5×)保存于2~8°C时,通常会有析出,如发现析出可将试剂置于37°C中温育、摇荡,使析出的试剂重新溶解。
1.2 使用前须用蒸馏水或超纯水将冰冻切片快速抗原修复液(5×)稀释5倍,配成抗原修复液(1×)。
2、抗原修复对于冰冻切片:用免疫染色洗涤液洗涤切片5min。将切片浸泡在抗原修复液(1×)中,室温孵育约5min。用免疫染色洗涤液洗涤3~5次,每次3-5min,以充分去除残留的SDS等试剂。随后即可进行封闭等后续的免疫染色步骤。
温馨提示:为了您的自身安全,使用试剂前,请做好防护,如穿实验服,带手套等。
Physical appearance:Liquid
Product presentation:
Frozen section rapid antigen repair solution (5×) contains SDS and other antigen repair reagents, pH value is about 7.4, can quickly and effectively remove the cross-linking between proteins caused by aldehyde fixing reagents, fully expose the antigen epitope in paraffin sections and other samples, thus greatly improving the immunostaining effect. It is especially suitable for rapid antigen repair of frozen sections, which can be completed by incubating at room temperature for 5min.
Operation process:
1. Reagent preparation
1.1 Frozen section rapid antigen repair solution (5×) is stored in 2~8°C, usually there will be precipitation, if found precipitation can be placed in 37°C warming, shaking, so that the reagent precipitated re-dissolve.
1.2 Before use, the frozen section rapid antigen repair solution (5×) should be diluted 5 times with distilled water or ultra-pure water, and the antigen repair solution (1×) should be prepared.
2. Antigen repair for frozen sections: wash sections with immunostaining washing solution for 5min. The slices were soaked in antigen repair solution (1×) and incubated at room temperature for about 5min. Wash with immunostaining detergent for 3 to 5 times, each time for 3-5min, to fully remove the remaining SDS and other reagents. Subsequent immunostaining steps such as blocking can then be performed.
Tips: For your own safety, please take good protection before using the reagent, such as wearing a lab coat, wearing gloves, etc.
| pH | 7.4 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Q752080 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Sterile-filtered 级别指南 → 查看 BioReagent 级别指南 → 查看 Sterile 级别指南 → 查看 Suitable for Immunohistochemistry(IHC) 级别指南 →