计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,无载体,生物活性 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Kex2蛋白酶是钙离子依赖性的蛋白水解酶,能特异性识别和切割Arg-Arg、Lys-Arg等双碱基氨基酸羧基端肽键,与胰蛋白酶不同,Kex2不能识别和切割单一碱性氨基酸即精氨酸或赖氨酸的羧基端肽键。在酵母体内Kex2蛋白酶负责加工killer toxin和α-factor的前体。Kex2蛋白酶是由毕赤酵母表达生产。酶的最适pH为9.0,最适温度为37℃。在偏酸性环境中稳定,稳定pH为5.0-6.0。Kex2蛋白酶活性不受常规的丝氨酸蛋白酶抑制剂如PMSF、TPCK所抑制。
Kex2蛋白酶推荐使用方法:
如果溶解后不立即使用,推荐溶解缓冲:20mM NaAc-HAc, 2 mM Ca2+, pH 5.0~5.5.
推荐酶切缓冲液:5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0.
推荐条件:
蛋白酶:融合蛋白质量比1:20~1:1000
Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α,factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0). The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.
Kex2 Protease Recommended Method:
Recommended reaction buffer: 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0. If it is not used immediately after dissolution, it is recommended to use 20mM NaAc-HAc, 2mM Ca2+ (pH 5.0~5.5) buffer to dissolve the lyophilized powder.
During the reaction, use 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0 as the reaction diluent.
Recommended conditions:
Protease: Fusion protein Ratio 1:20~1:1000.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R141093 | |
| 分析证书 | R141093 | |
| 分析证书 | R141093 | |
| 分析证书 | R141093 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 EnzymoPure™ 级别指南 →