计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥97% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
RIP1/RIP3/MLKL activator 1 (Compound 6i) is a potent anti-glioma agent. RIP1/RIP3/MLKL activator 1 induces necroptosis through RIP1/RIP3/MLKL pathway. RIP1/RIP3/MLKL activator 1 exerts acceptable BBB permeability
In Vitro
RIP1/RIP3/MLKL activator 1 (Compound 6i) (96 h) shows antiproliferative activities in human glioma cell lines. RIP1/RIP3/MLKL activator 1 (0-4 µM, 0-72 h) exhibits remarkable antiproliferative activity for U251 cells in a time- and concentration-dependent manner. RIP1/RIP3/MLKL activator 1 (10 µM, 0-72 h) shows acceptable stability. RIP1/RIP3/MLKL activator 1 (0-2 µM, 24 h) effectively inhibits the migration of U251 cells. RIP1/RIP3/MLKL activator 1 induces necroptosis through RIP1/RIP3/MLKL pathway, and induces mitochondrial depolarization in U251 cells. RIP1/RIP3/MLKL activator 1 could not induce apoptosis in U251 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: A172, LN229, U87, U251 and L02 cell lines Concentration: 0-4 µM for U251 cells Incubation Time: 96 h; 24, 48, and 72 h for U251 cells Result: Showed antiproliferative activity with IC 50 values of 3.03 ± 0.70, 1.78 ± 0.79, 1.22 ± 0.89, 0.94 ± 0.45, and 0.99 ± 0.46 µM against A172, LN229, U87, U251 and L02 cells, respectively. Time- and concentration-dependently inhibited the growth in U251 cells. Western Blot AnalysisCell Line: U251 Concentration: 0, 0.5, 1, 2, and 4 µM Incubation Time: 24 or 48 h Result: Concentration-dependently upregulated the expression of p-RIP1, RIP1, p-RIP3, RIP3, p-MLKL, and MLKL at 24 or 48 h.
In Vivo
RIP1/RIP3/MLKL activator 1 (Compound 6i) (2.50 ng/tail; i.v.; 48 h) inhibits U251 cell proliferation in vivo and exerts acceptable BBB permeability . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Zebrafish wide-type AB strain; 200 CM-DiI labeled U251 cells were transplanted into yolk sac of each wild-type zebrafish embryos at 2 dpf (2 days postfertilization) Dosage: 2.50 ng/tail Administration: Microinjection; 48 h Result: Remarkably reduced the U251 xenografts fluorescence intensity.
Form:Solid
IC50& Target:RIP1, RIP3, MLKL
规格
别名
RIP1/RIP3/MLKL 激活剂 1
规格或纯度
≥97%
英文名称
RIP1/RIP3/MLKL activator 1
生化机理
RIP1/RIP3/MLKL 激活剂 1（化合物 6i）是一种有效的抗胶质瘤药物。RIP1/RIP3/MLKL 激活剂 1 可通过 RIP1/RIP3/MLKL 通路诱导坏死。RIP1/RIP3/MLKL 激活剂 1 具有可接受的 BBB 通透性。
储存条件
2-8°C储存
运输条件
低温运输
纯度
≥97%
名称和识别符
分子类型
小分子
Smiles
CC1=C(C(C=C2C1=CC=C3[C@]4(CC[C@]5(CC[C@@](C(NCC6=CN(N=N6)CC7=CC=C(C=C7)C(C)(C)C)=O)(CC5[C@@]4(CC[C@@]23C)C)C)C)C)=O)O
分子量
676.93
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 100 mg/mL (147.73 mM; Need ultrasonic)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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