计算溶液所需的质量、体积或浓度。
BioReagent,即用型,无RNA酶,无菌 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本保存液是一种液态、无毒的血液 RNA 保存试剂,通过与 RNA 形成复合物从而保护 RNA完整性,同时抑制 RNase 活性。本试剂能立即稳定新鲜血液中的 RNA,含有本试剂的人血液样品可在 2-8℃保存 5 天,或在-20℃条件下至少保存三个月,含有本试剂的哺乳动物血液样品可在 15-25℃保存 2 天,2-8℃保存 7 天,或在-20℃ 条件下至少保存六个月,RNA 不会出现明显降解。后续使用血液总RNA提取试剂盒结合优化流程,可从保存于血液样本RNA保存液的血液中快速提取总 RNA。
产品特点:
1) 保护新鲜血液中 RNA 的完整性,不被降解。
2) 操作简便,适用于大量血液样本的集中保存。
3) 配合阿拉丁生产的血液总RNA提取试剂盒 (产品目录号:R1125010) 提取血液 RNA,保证核酸得率和纯度。
a) 使用前请确保血液样本 RNA 保存液储存于室温。取新鲜抗凝全血按 1:3 的比例加入血液样本 RNA 保存液(如取 300μl 健康人新鲜抗凝全血,加入 900μl 血液样本 RNA 保存液或 100μl 哺乳动物新鲜抗凝全血,加入 300 μl 血液样本 RNA 保存液)。
b) 立即盖上管盖,上下颠倒混匀 8-10 次。含有血液样本 RNA 保存液的人血液样品可在2-8℃保存 5 天,或在-20℃条件下至少保存三个月;含有血液 RNA 保存液的哺乳动物血液样品可在 15-25℃保存 2 天,2-8℃保存 7 天,或在-20℃条件下至少保存六个月。
注意事项:
a) 保存血液样品时,请立即在新鲜抗凝全血中加入 3 倍体积的血液样本 RNA 保存液。如需处理更大量的血液样本,请选择合适规格的耗材进行操作。
b) 含有血液样本 RNA 保存液的血液样品必须在室温放置 2 h 以上,以使血液样品充分溶解于保存液中。该步骤在样本低温保存前后都可进行。
This preservation solution is a liquid, non-toxic reagent for preserving blood RNA. It protects RNA integrity by forming a complex with RNA and simultaneously inhibits RNase activity. This reagent immediately stabilizes RNA in fresh blood. Human blood samples containing this reagent can be stored at 2-8℃ for 5 days or at -20℃ for at least 3 months. Mammalian blood samples containing this reagent can be stored at 15-25℃ for 2 days, at 2-8℃ for 7 days, or at -20℃ for at least 6 months, with no significant RNA degradation observed. For subsequent use, total RNA can be rapidly extracted from blood preserved in this blood sample RNA preservation solution using a total blood RNA extraction kit combined with an optimized protocol.
Product Features:
1. Preserves the integrity of RNA in fresh blood and prevents RNA degradation.
2. Simple to operate, suitable for centralized preservation of a large number of blood samples.
3. Cooperate with the Blood Total RNA Extraction Kit (Catalog No.: R1125010) produced by Aladdin to extract blood RNA, so as to ensure the yield and purity of nucleic acids.
a) Before use, ensure the Blood Sample RNA Preservation Solution is stored at room temperature. Take fresh anticoagulated whole blood and add it to the Blood Sample RNA Preservation Solution at a ratio of 1:3. For example: Add 300 μl of fresh anticoagulated whole blood from a healthy human to 900 μl of Blood Sample RNA Preservation Solution; Or add 100 μl of fresh anticoagulated whole blood from a mammal to 300 μl of Blood Sample RNA Preservation Solution.
b) Immediately cap the tube and invert it up and down 8-10 times to mix thoroughly. Human blood samples containing the Blood Sample RNA Preservation Solution can be stored at 2-8℃ for 5 days or at -20℃ for at least 3 months; Mammalian blood samples containing the Blood RNA Preservation Solution can be stored at 15-25℃ for 2 days, at 2-8℃ for 7 days, or at -20℃ for at least 6 months.
Precautions:
a) When preserving blood samples, immediately add 3 volumes of Blood Sample RNA Preservation Solution to fresh anticoagulated whole blood. If processing a larger volume of blood samples, select appropriately sized consumables for the operation.
b) Blood samples containing Blood Sample RNA Preservation Solution must be left at room temperature for at least 2 hours to ensure the blood samples are fully dissolved in the preservation solution. This step can be performed either before or after the samples are stored at low temperatures.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R751648 |
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