Name: 钌红染色液 (0.01%)
SKU: R774810
Availability: InStock

钌红染色液 (0.01%)

CAS: 11103-72-3 货号: R774810 分子式: H₄₂Cl₆N₁₄O₂Ru₃ 分子量: 786.35 EC号: 802-133-7 PubChem CID: 16218584

概述规格名称和识别符高级数据质检证书(CoA,COO,BSE/TSE 和分析图谱) 化学和物理性质安全和危险性（GHS）此产品的引用文献技术文档和文章溶液计算器

概述

钌红染色液 (0.01%) 由钌红与去离子水配制，为聚阳离子染料，可结合酸性多糖，常用于植物粘胶类物质染色。十字花科、车前科植物（拟南芥等）种皮粘液质以果胶（鼠李半乳糖醛酸聚糖）为主要组分，是细胞壁多糖研究的经典模型。种子吸水后粘液质释放，经本染色液染色可清晰观察粘液质表型；也可用于植物病害组织中粘胶物质检测，健康组织细胞壁被染成红色，病原菌降解粘胶的病组织不显色。本产品仅限科研使用，不得用于临床诊断。

自备材料：

1、蒸馏水、显微镜、镊子、吸管、恒温箱或水浴锅

2、拟南芥种子、植物组织(正常组织或病害组织)等样品

操作步骤(仅供参考)：

1、植物组织粘胶质的染色：

①将植物组织切成薄片，如果需要观察病菌分解粘胶类物质的作用，则切片应包括病害部分和正常部分。

②切片滴加钌红染色液避光染色 30～60 分钟。

③水中冲洗干净。

④滴加甘油或甘油明胶封片剂，盖盖玻片，显微镜镜检。

2、种子粘胶质的染色：

①将一定数量的成熟种子放入离心管或平皿中，加入适当体积的蒸馏水，湿润种子，并使其充分吸收水分，根据种子大小，时间为 1～12 小时不等。

②在不扰动种子的情况下尽可能多地吸除水分。

③加入钌红染色液并充分接触种子，避光染色 60 分钟。

④吸除染色液，加入少量蒸馏水，将种子转移到凹槽载玻片上观察染色情况。

实验结果：

植物病虫害的组织中，不被染色，正常组织呈红色。

植物种子中，外层粘胶质呈浅粉红色，易脱落；内层粘胶质呈深红色，呈射线状结构，与种子表面结合紧密，不易被除去。

注意事项：

1、钌红染色液见光易变质，因此染色时建议避光进行。

2、由于干扰物质的影响，钌红不染色并不代表不存在粘胶质。

3、为了您的安全和健康，请穿实验服并戴一次性手套操作。

4、试剂开封后请尽快使用，以防影响后续实验效果。

Ruthenium Red Stain (0.01%) is formulated with ruthenium red and deionized water. As a polycationic dye, it binds to acidic polysaccharides and is commonly used for staining plant mucilaginous substances. The seed coat mucilage of Brassicaceae, Plantaginaceae (e.g., Arabidopsis thaliana) consists mainly of pectin (rhamnogalacturonan), serving as a classic model for cell wall polysaccharide research. Upon seed imbibition, mucilage is released and can be clearly visualized after staining with this solution. It is also applicable for detecting mucilaginous substances in diseased plant tissues: cell walls of healthy tissues stain red, while diseased tissues with pathogen-degraded mucilage remain unstained. For research use only. Not for clinical diagnosis.

Materials Required but Not Provided:

1、Distilled water, microscope, tweezers, pipette, incubator or water bath

2、Arabidopsis thaliana seeds, plant tissues (healthy or diseased tissues), and other samples

Operating Procedure (For Reference Only):

1、Staining of Mucilage in Plant Tissues:

①Cut the plant tissue into thin sections. If it is necessary to observe the decomposition of mucilaginous substances by pathogens, the sections should include both diseased and healthy portions.

②Add drops of ruthenium red staining solution to the sections and stain for 30–60 minutes protected from light.

③Rinse thoroughly with water.

④Add glycerol or glycerol gelatin mounting medium, cover with a coverslip, and examine under a microscope.

2、Staining of Seed Mucilage:

①Place a certain number of mature seeds in a centrifuge tube or Petri dish, add an appropriate volume of distilled water to moisten the seeds, and allow them to fully absorb water. Depending on the seed size, the time ranges from 1 to 12 hours.

②Remove as much water as possible without disturbing the seeds.

③Add ruthenium red staining solution and ensure full contact with the seeds. Stain for 60 minutes protected from light.

④Remove the staining solution, add a small amount of distilled water, and transfer the seeds to a concave microscope slide to observe the staining results.

Experimental Results:

Plant tissues affected by diseases and pests are not stained, while healthy tissues appear red.

In plant seeds, the outer mucilage is light pink and easily detached; the inner mucilage is dark red, exhibits a radial structure, is tightly bound to the seed surface, and is not easily removed.

Precautions:

1、Ruthenium red staining solution deteriorates easily upon exposure to light. Therefore, staining should preferably be performed protected from light.

2、Due to the influence of interfering substances, the absence of ruthenium red staining does not necessarily indicate the absence of mucilage.

3、For your safety and health, please wear a laboratory coat and disposable gloves during operation.

4、Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.

规格

规格或纯度

BioReagent, 生物染色剂, 0.01%

稳定性与储存

Store at room temperature long term (12 months). Store in the dark.

英文名称

Ruthenium Red Stain (0.01%)

储存条件

避光,室温

运输条件

常规运输

产品线

生物产品

名称和识别符

EC号

802-133-7

分子类型

生物试剂/缓冲液

Isomeric SMILES

N.N.N.N.N.N.N.N.N.N.N.N.N.N.O.O.[Cl-].[Cl-].[Cl-].[Cl-].[Cl-].[Cl-].[Ru+2].[Ru+2].[Ru+2]

PubChem CID

16218584

分子量

786.35

高级数据

化学和物理性质

敏感性

Light-sensitive

熔点

>500°C

安全和危险性（GHS）

WGK Germany

个人防护装备

Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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批号(Lot Number)	证书类型	货号
ZJ26F0434140	分析证书	R774810

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