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RPMI-1640 (1×) 培养基
有货
为什么选择此级别
BioReagent,无菌过滤,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
RPMI 1640培养基以研发地点罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute. RPMI）命名，1640为培养基代号。它是McCoy's 5A培养基的改进型，使用碳酸氢盐缓冲系统。RPMI 1640培养基最初开发用于人白血病细胞的悬浮或单层培养，后来被发现也适用于多种哺乳动物细胞，包括HeLa、Jurkat、MCF-7、PC-12、PBMC、星形胶质细胞和癌细胞，尤其适用于悬浮细胞的培养，是使用最为广泛的培养基之一。
RPMI 1640培养基与其它培养基的区别在于含有还原型谷胱甘肽和高浓度的维生素。RPMI 1640培养基含有EMEM和DMEM中没有的生物素、维生素B12和对氨基苯甲酸，以及高浓度的氯化胆碱和肌醇。
本产品含有：D-葡萄糖、酚红、L-谷氨酰胺。 不含有：丙酮酸钠、HEPES。
生产工艺：
本产品使用注射用水（Water-For-Injection）配制，生产程序参考GMP管理规定，并在净化车间中完成。公司已取得ISO9001质量体系认证。
产品优势：
注射用水配制，符合cGMP规定生产，工艺稳定，批差异小。
原料均选用自产或者经过严格检测和纯化的低内毒素高纯药用级别原料。
严格的质量检测程序，远高于同行业要求指标，产品性能突出，指标媲美进口。
热原（内毒素）水平低，减少外源内毒素干扰。
进口材质无菌包装瓶，符合cGMP管理规定，优秀的密闭性及内部涂层保护，极大的保证了产品的稳定性。
注意事项：
本培养液4ºC保存时应避免靠近冰箱壁，以免低温导致沉淀析出。如果有沉淀析出，室温摇晃片刻，再放置在4ºC冰箱过夜或更长时间，待沉淀消失后再使用。
若使用过程中发现培养液浑浊、沉淀等现象，则不能继续使用。
用于检测新生蛋白质的合成时，不建议添加血清。
使用前推荐37ºC预热或在室温平衡后再使用。
开瓶后不要长时间暴露在空气和强光下，以保持培养液的稳定性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
RPMI 1640 medium is named after Roswell Park Memorial Institute (RPMI), and 1640 is the medium code number. It is a modified version of McCoy’s 5A medium with a bicarbonate‑buffered system. Originally developed for suspension or monolayer culture of human leukemia cells, RPMI 1640 medium has proven suitable for a wide range of mammalian cells, including HeLa, Jurkat, MCF‑7, PC‑12, PBMC, astrocytes and cancer cells. It is especially favorable for suspension cell culture and ranks among the most widely‑used cell culture media.
Different from other media, RPMI 1640 medium contains reduced glutathione and high‑level vitamins. It comprises biotin, vitamin B12 and para‑aminobenzoic acid which are absent in EMEM and DMEM, as well as high concentrations of choline chloride and inositol.
Components included in this product: D‑glucose, phenol red, L‑glutamine.
Components excluded: sodium pyruvate, HEPES.
Manufacturing Process
This product is formulated with Water‑For‑Injection (WFI). Manufacturing procedures follow GMP guidelines and are completed in clean workshops. Our company holds ISO 9001 quality system certification.
Product Advantages
Formulated with Water‑For‑Injection and manufactured in compliance with cGMP requirements, delivering stable processes and minimal batch‑to‑batch variation.
Raw materials are either self‑produced or strictly tested and purified high‑purity pharmaceutical‑grade materials with low endotoxin levels.
Rigorous quality control procedures with stricter specifications than general industrial standards, providing comparable performance to imported counterparts.
Low pyrogen (endotoxin) level to minimize interference from exogenous endotoxins.
Sterile bottles made of imported materials complying with cGMP requirements. Superior sealing performance and inner‑coating protection greatly guarantee product stability.
Precautions
When stored at 4 °C, keep this medium away from refrigerator walls to avoid precipitation induced by excessive low temperature. If precipitate occurs, shake gently at room temperature, then store at 4 °C overnight or longer until precipitate dissolves prior to use.
Discard the medium in case of turbidity or visible precipitate during usage.
Serum addition is not recommended when assaying nascent protein synthesis.
Warm up to 37 °C or equilibrate to room temperature before use.
Avoid prolonged exposure to air and intense light after bottle opening to maintain medium stability.
For research use only by qualified professionals. Not for clinical diagnosis or therapeutic applications. Not for food or pharmaceutical purposes. Do not store in residential premises.
Wear laboratory coat and disposable gloves for your safety and health.
规格
别名
1640培养基 | RPMI1640培养基
英文别名
Roswell Park Memorial Institute
规格或纯度
无菌过滤, BioReagent, 用于细胞培养
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
英文名称
RPMI-1640 Medium (1×)
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
培养基
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939570 |
分析证书 | R778408 |
| ZJ25F0521934 |
分析证书 | R778408 |
技术文档和文章
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