1. Measured by its binding ability in a functional ELISA. When Recombinant Human MCAM/CD146 is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human Galectin-3. The concentration of Recombinant Human Galectin-3 that produces 50% of the optimal binding response is 0.25‑1.25 μg/mL. 2. Immobilized Recombinant Human MCAM/CD146 Protein (rp143805) at 1 μg/mL can bind Imaprelimab (anti-CD146) (Ab175560) with the EC50 for this effect is 38.86 ng/mL.
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
1. Measured by its binding ability in a functional ELISA. When Recombinant Human MCAM/CD146 is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human Galectin-3. The concentration of Recombinant Human Galectin-3 that produces 50% of the optimal binding response is 0.25‑1.25 μg/mL. 2. Immobilized Recombinant Human MCAM/CD146 Protein (rp143805) at 1 μg/mL can bind Imaprelimab (anti-CD146) (Ab175560) with the EC50 for this effect is 38.86 ng/mL.
80 - 110 kDa, under reducing conditions; 80 - 110 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human MCAM/CD146 Protein (rp143805) - Protein Bioactivity Immobilized Recombinant Human MCAM/CD146 Protein (rp143805) at 1 μg/mL can bind Imaprelimab (anti-CD146) (Ab175560) with the EC50 for this effect is 38.86 ng/mL.
Recombinant Human MCAM/CD146 Protein (rp143805) - SDS-PAGE 3 μg/lane of Recombinant Human MCAM/CD146 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 80 - 110 kDa.