基本描述

产品名称	Recombinant Human CD80 Protein
别名	重组人 CD80 蛋白
英文别名	Activation B7-1 antigen \| B7 \| BB1 \| CTLA-4 counter-receptor B7.1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity >95% SDS-PAGE. Molecular weight informat The protein migrates as 65-90 kDa under reducing condition due to glycosylation. Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
生物活性	Measured by its binding activity of recombinant human CD80 and recombinant human CD86. The EC50 for this effect is less than 0.412μg/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	Human
氨基酸	35-242 aa
序列	VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIW PEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEEL NAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFN WNTTKQEHFPDN
纯度	＞95% (SDS-PAGE)
蛋白标签	His
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	P33681
来源	重组表达
预测分子量	25.5 kDa

AI解读

图片

Protein Bioactivity: Recombinant Human CD80 Protein (rp144122)

Recombinant Human CD80 Protein (rp144122) - Protein Bioactivity
Measured by its binding activity of recombinant human CD80 and recombinant human CD86. The EC₅₀ for this effect is less than 0.412 μg/mL.

SDS-PAGE: Recombinant Human CD80 Protein (rp144122)

Recombinant Human CD80 Protein (rp144122) - SDS-PAGE
2.5 μg/lane of Recombinant Human CD80 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 50.2 kDa.

产品规格参数

应用	Cell culture; Activity Assays
物理形态	冻干(Lyophilized)
储存缓冲液	PBS, pH7.4, containing 5% trehalose.
复溶	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not Vortex.
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	避免反复冻融循环。在 2-8 ℃ 下保存一个月。分装后在 -80 ℃ 下保存 12 个月。
分子类型	蛋白质

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找到2个结果

批号(Lot Number)	证书类型	日期	货号
ZJ23F0700615	分析证书	23-07-20	rp144122
ZJ23F0700614	分析证书	23-07-20	rp144122

参考文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e]
2. Vicente Rabaneda EF, Herrero-Beaumont G, Castañeda S. (2013) Update on the use of abatacept for the treatment of rheumatoid arthritis.. Expert Rev Clin Immunol, 9 (7): (599-621). [PMID:23899231] [10.1021/op500134e]
3. Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK, Ledbetter JA. (1991) CTLA-4 is a second receptor for the B cell activation antigen B7.. J Exp Med, 174 (3): (561-9). [PMID:1714933] [10.1021/op500134e]
4. Selvakumar, A A and 5 more authors.. (1992) Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7.. Immunogenetics, [PMID:1377173]
5. Freeman, G J GJ and 8 more authors.. (1991) Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.. The Journal of experimental medicine, (1): [PMID:1714935]
6. Freeman, G J GJ and 5 more authors.. (1989) B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2794510]
7. Lanier, L L LL and 7 more authors.. (1995) CD80 (B7) and CD86 (B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:7527824]
8. Vandenborre, K K and 5 more authors.. (1999) Interaction of CTLA-4 (CD152) with CD80 or CD86 inhibits human T-cell activation.. Immunology, [PMID:10583602]
9. Ikemizu, S S and 7 more authors.. (2000) Structure and dimerization of a soluble form of B7-1.. Immunity, [PMID:10661405]
10. Stamper, C C CC and 9 more authors.. (2001) Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses.. Nature, (29): [PMID:11279502]
11. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]
12. Gottlieb, Alice B AB and 8 more authors.. (2004) Evaluation of safety and clinical activity of multiple doses of the anti-CD80 monoclonal antibody, galiximab, in patients with moderate to severe plaque psoriasis.. Clinical immunology (Orlando, Fla.), [PMID:15093549]
13. Czuczman, Myron S MS and 13 more authors.. (2005) Phase I/II study of galiximab, an anti-CD80 antibody, for relapsed or refractory follicular lymphoma.. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, (1): [PMID:15994148]
14. and Kremer, Joel M JM.. (2005) Selective costimulation modulators: a novel approach for the treatment of rheumatoid arthritis.. Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, [PMID:16357751]
15. Hervey, Pauline S PS and Keam, Susan J SJ.. (2006) Abatacept.. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, [PMID:16573350]
16. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997]
17. Weyand, Cornelia M CM and Goronzy, Jörg J JJ.. (2006) T-cell-targeted therapies in rheumatoid arthritis.. Nature clinical practice. Rheumatology, [PMID:16932686]
18. and Scheinfeld, Noah N.. (2006) Abatacept: A review of a new biologic agent for refractory rheumatoid arthritis for dermatologists.. The Journal of dermatological treatment, [PMID:16971318]
19. Tedesco Silva, Helio H, Pinheiro Machado, Paula P, Rosso Felipe, Claudia C and Medina Pestana, Jose Osmar JO.. (2006) Immunotherapy for De Novo renal transplantation: what's in the pipeline?. Drugs, [PMID:16978033]
20. Vincenti, Flavio F and Luggen, Michael M.. (2007) T cell costimulation: a rational target in the therapeutic armamentarium for autoimmune diseases and transplantation.. Annual review of medicine, [PMID:17020493]
21. Nogid, Anna A and Pham, David Q DQ.. (2006) Role of abatacept in the management of rheumatoid arthritis.. Clinical therapeutics, [PMID:17212998]
22. Leonard, J P JP and 14 more authors.. (2007) A phase I/II study of galiximab (an anti-CD80 monoclonal antibody) in combination with rituximab for relapsed or refractory, follicular lymphoma.. Annals of oncology : official journal of the European Society for Medical Oncology, [PMID:17470451]
23. Kakoulidou, M M, Giscombe, R R, Zhao, X X, Lefvert, A K AK and Wang, X X.. (2007) Human Soluble CD80 is generated by alternative splicing, and recombinant soluble CD80 binds to CD28 and CD152 influencing T-cell activation.. Scandinavian journal of immunology, [PMID:17953528]
24. Reynolds, Jennifer J, Shojania, Kam K and Marra, Carlo A CA.. (2007) Abatacept: a novel treatment for moderate-to-severe rheumatoid arthritis.. Pharmacotherapy, [PMID:18041889]
25. Maxwell, Lara J LJ and Singh, Jasvinder A JA.. (2010) Abatacept for rheumatoid arthritis: a Cochrane systematic review.. The Journal of rheumatology, [PMID:20080922]
26. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
27. Kleinpeter, Patricia and 20 more authors.. (2019) By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1.. Journal of virology, (1): [PMID:30918073]

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货号 (SKU)	包装规格	是否现货
rp144122-25μg	25μg	现货
rp144122-100μg	100μg	现货
rp144122-1mg	1mg	期货

Recombinant Human CD80 Protein

库存信息

库存信息

库存信息

基本描述

AI解读

图片

产品规格参数

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关文档

参考文献

溶液计算器

摩尔浓度计算器

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