1、Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA3 is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 ‑ 25 ng/mL. 2、Immobilized Recombinant Human EphA3 Protein (rp145609) at 1.0 μg/mL can bind Ifabotuzumab (anti-EphA3) (Ab191893) with the EC50 is 13.82 ng/mL.
1、Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA3 is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 ‑ 25 ng/mL. 2、Immobilized Recombinant Human EphA3 Protein (rp145609) at 1.0 μg/mL can bind Ifabotuzumab (anti-EphA3) (Ab191893) with the EC50 is 13.82 ng/mL.
65 - 80 kDa, under reducing conditions; 60 - 75 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
biological_products
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human EphA3 Protein (rp145609) - Protein Bioactivity Immobilized Recombinant Human EphA3 Protein (rp145609) at 1.0 μg/mL can bind Ifabotuzumab (anti-EphA3) (Ab191893) with the EC50 is 13.82 ng/mL.
Recombinant Human EphA3 Protein (rp145609) - SDS-PAGE 2 μg/lane of Recombinant Human EphA3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 65 - 80 kDa under reducing conditions and 60 - 75 kDa under non-reducing conditions.