级别和纯度: 无动物源?无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。无叠氮钠?无叠氮(Azide Free)—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥95%(SDS-PAGE)
Accession #
P98172
蛋白标签
C-hFc & His
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Immobilized Recombinant Human EphB3 Chimera Protein (rp184031) at 2.0 μg/mL can bind Recombinant Human Ephrin-B1 Protein (rp145638) with the ED50 of 139.2 ng/mL.
Purity ≥95% (SDS-PAGE) Endotoxin level <0.1 EU/μg Function Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Immobilized Recombinant Human EphB3 Chimera Protein (rp184031) at 2.0 μg/mL can bind Recombinant Human Ephrin-B1 Protein (rp145638) with the ED50 of 139.2 ng/mL.
55 - 65 kDa, under reducing conditions; 55 - 60 &120 - 140 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human Ephrin-B1 Protein (rp145638) - Protein Bioactivity Immobilized Recombinant Human EphB3 Chimera Protein (rp184031) at 2.0 μg/mL can bind Recombinant Human Ephrin-B1 Protein (rp145638) with the ED50 of 139.2 ng/mL.
Recombinant Human Ephrin-B1 Protein (rp145638) - SDS-PAGE 3 μg/lane of Recombinant Human Ephrin-B1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 - 65 kDa under reducing condition and 55 - 60 &120 - 140 kDa under non-reducing condition.