Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED₅₀ for this effect is typically <0.5ng/mL.
>95% as determined by SDS-PAGE and Coomassie blue staining
Function
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED₅₀ for this effect is typically <0.5ng/mL.
Lyophilized from a sterile solution containing 50 mM Tris.Cl, pH7.5, 150mM NaCl.
复溶
After receiving lyophilized powder protein, centrifuge first, and then r econstitute at 100µg/mL in sterile PBS.
储存条件
-20°C储存
运输条件
超低温运输
稳定性与储存
在适当的储存和处理条件下，自收到产品之日起 12 个月内保持稳定。避免反复冻融循环。
图片
Recombinant Human FGF1 Protein (rp145907)-Protein Bioactivity Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED50 for this effect is typically <0.5ng/mL.
Recombinant Human FGF1 Protein (rp145907)-SDS-PAGE 3μg/lane of Recombinant Human FGF1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.3 kDa.
ZJ23F0400116
危险品化学品经营许可证（带存储）