1、Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 5.71 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1(rp147252). 2、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-6 Protein (rp147288) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 131.2 ng/mL.
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
生物活性
1、Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 5.71 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1(rp147252). 2、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-6 Protein (rp147288) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 131.2 ng/mL.
30 - 37 kDa, under reducing conditions; 27 - 34 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human IGFBP-6 Protein (rp147288) - SDS-PAGE 2 μg/lane of Recombinant Human IGFBP-6 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 30 - 37 kDa under reducing condition and 27 - 34 kDa under non-reducing condition.
Recombinant Human IGFBP-6 Protein (rp147288) - Protein Bioactivity Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 5.71 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1(rp147252).
Recombinant Human IGFBP-6 Protein (rp147288) - Protein Bioactivity Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-6 Protein (rp147288) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 131.2 ng/mL.
ZJ25F0420306
危险品化学品经营许可证（带存储）