Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK.
生物活性
The cell proliferation assay using TF-1 human erythroleukemic cells has an ED50 value of 0.05-0.3 ng/mL.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
常规运输
稳定性与储存
长期储存-20~-80℃(1年);复溶后建议分装,避免反复冻融。
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Recombinant Human IL-3 Protein (rp147548) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.21 ng/mL.
Recombinant Human IL-3 Protein (rp147548) - SDS-PAGE
2 μg/lane of Recombinant Human IL-3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 20 - 30 kDa.