Measured by the ability of the immobilized protein to support the adhesion of BT‑20 human breast cancer cells. When 5 x 10^4 cells/well are added to rhJAML coated plates (10 µg/mL, 100 µL/well), approximately 55%-75% will adhere after 1 hour at 37° C. Similar results were obtained using HeLa, A549, and T84 cell-lines. Optimal dilutions should be determined by each laboratory for each application.
May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells.
规格
产品名称
Recombinant Human AMICA Protein
别名
连接粘附分子样蛋白重组蛋白
英文别名
adhesion molecule, interacts with CXADR antigen 1 | AMICA | AMICA1 | CREA7-1 | Gm638 | JAML | CREA7-4 | Dendritic cell-specific protein CREA7-1 | dendritic-cell specific protein CREA7-1 | dendritic-cell specific protein CREA7-4 | Junctional adhesion molec
Measured by the ability of the immobilized protein to support the adhesion of BT‑20 human breast cancer cells. When 5 x 10^4 cells/well are added to rhJAML coated plates (10 µg/mL, 100 µL/well), approximately 55%-75% will adhere after 1 hour at 37° C. Similar results were obtained using HeLa, A549, and T84 cell-lines. Optimal dilutions should be determined by each laboratory for each application.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human AMICA Protein (rp147886) - SDS-PAGE 2 μg/lane of Recombinant Human AMICA Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 32 - 40 kDa under reducing condition and 30 - 38 kDa under non-reducing condition.