ActiBioPure™,His标签,无动物源,无叠氮钠,无载体,生物活性 级 提供 ≥97%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity
>97% SDS-PAGE.
Actual molecular weight
25.7 kDa
Molecular weight informat
~ 25.7 kDa (monomer). Human lipocalin-2 (aa 1-198) is fused at the C-terminus to a polyhistidine tag.
Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Recombinant Human Lipocalin-2/NGAL Protein (rp148318) - Protein Bioactivity
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2/NGAL Protein (rp148318). Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3.
Iron III (Fe3+): Aladdin, Cat# I115411
2,3,Dihydroxybenzoic Acid (DHBA): Aladdin, Catalog # D154867
Recombinant Human Lipocalin-2/NGAL Protein (rp148318) - SDS-PAGE
3 μg/lane of Recombinant Human Lipocalin-2/NGAL was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 24.6 kDa.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0901001 |
分析证书 | rp148318 |
| ZJ23F0901002 |
分析证书 | rp148318 |
| ZJ23F0901003 |
分析证书 | rp148318 |
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