Recombinant Human MIF Protein

无动物源

无载体

生物活性

ActiBioPure™

无叠氮钠

≥95%(SDS-PAGE)

功能和特点

表达系统: E. coli
Accession #: P14174
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp148808-10μg	10μg	现货
rp148808-50μg	50μg	现货
rp148808-100μg	100μg	现货
rp148808-1mg	1mg	期货

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点击查看蛋白信息: MIF

Accession#:P14174 (5) Gene ID:4282 (5) MIF (5)

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基本描述

产品名称	Recombinant Human MIF Protein
别名	重组人MIF蛋白 \| 重组人类 MIF 蛋白
英文别名	EC 5.3.2.1 \| EC 5.3.3.12 \| GIFmacrophage migration inhibitory factor \| GLIF \| Glycosylation-inhibiting factor \| L-dopachrome isomerase \| L-dopachrome tautomerase \| macrophage migration inhibitory factor (glycosylation-inhibiting factor) \| MIF \| MMIF \| Phe
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. Function Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
生物活性	Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	1-115 aa
序列	MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
纯度	≥95%(SDS-PAGE)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	P14174
来源	重组表达
预测分子量	12.5 kDa
SDS-PAGE	12.5 kDa

AI解读

图片

Protein Bioactivity: Recombinant Human MIF protein (rp148808)

Recombinant Human MIF protein (rp148808)-Protein Bioactivity
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.

SDS-PAGE: Recombinant Human MIF protein (rp148808)

Recombinant Human MIF protein (rp148808)-SDS-PAGE
Recombinant Human MIF protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 12.5 kDa.

产品规格参数

应用	Active,SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	PBS, pH 7.4 with 5% trehalose and 0.02% Tween-80
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤﹣20℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冰柜，避免反复冻融循环。自收到之日起 12 个月，-20 至 -70 °C。复溶后 1 个月，无菌条件下 2 至 8 °C。3 个月，复溶后在无菌条件下 -20 至 -70 °C。
分子类型	蛋白质

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找到3个结果

批号(Lot Number)	证书类型	日期	货号
ZJ23F0600322	分析证书	24-03-26	rp148808
ZJ23F0600323	分析证书	24-03-26	rp148808
ZJ23F0600324	分析证书	24-03-26	rp148808

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参考文献

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Recombinant Human MIF Protein

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基本描述

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