级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。无叠氮钠?无叠氮（Azide Free）—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥95%(SDS-PAGE)
Accession #
P15941-11
蛋白标签
C-hFc & His
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Measured by its ability to increase beta-catenin levels in the cytoplasm and nucleus of HCT116 human colon adenocarcinoma cells. 0.01-1 ng/mL of Recombinant Human MUC-1 can effectively increase beta-catenin levels.
Purity ≥95% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
规格
产品名称
Recombinant Human MUC-1 Protein
别名
粘蛋白1(MUC1)重组蛋白 | 重组人类 MUC-1 蛋白
英文别名
ADMCKD | ADMCKD1 | Breast carcinoma associated antigen DF3 | Breast carcinoma-associated antigen DF3 | CA 15-3 | CA15 3 | CA15 3 antigen | CA15-3 | CA15.3 | Cancer antigen 15-3 | Carcinoma associated mucin | Carcinoma-associated mucin | CD 227 | CD227 | D
Measured by its ability to increase beta-catenin levels in the cytoplasm and nucleus of HCT116 human colon adenocarcinoma cells. 0.01-1 ng/mL of Recombinant Human MUC-1 can effectively increase beta-catenin levels.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human MUC-1 Protein (rp148996) - Protein Bioactivity Measured by its ability to increase beta-catenin levels in the HCT116 human colon adenocarcinoma cells. 0.01 -1.0 ng/mL of Recombinant Human MUC-1 Protein (rp148996) can effectively increase beta-catenin levels.
Recombinant Human MUC-1 Protein (rp148996) - SDS-PAGE 3 μg/lane of Recombinant Human MUC-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 - 65 kDa.
ZJ24F1214952
危险品化学品经营许可证（带存储）