Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity.
生物活性
Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL .
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 运输。发货后,分装并储存于 -20°C 或 -80°C。避免反复冻融循环:0.0154% DTT、0.316% Tris HCl、10% 甘油、0.58% 氯化钠
图片
Binding Activity: Recombinant Human Myoglobin Protein (rp149044)
Immobilized Recombinant Human Myoglobin Protein (rp149044) at 2.0 μg/mL can bind Recombinant Myoglobin Antibody (Ab008056) with the EC₅₀ of 1.3 μg/mL.
SDS-PAGE: Recombinant Human Myoglobin Protein (rp149044)
2 μg/lane of Recombinant Human Myoglobin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 18 kDa.