Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
Function This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
规格
产品名称
Recombinant Human P4HB Protein
别名
重组人P4HB蛋白 | 重组人蛋白二硫键异构酶蛋白
英文别名
Cellular thyroid hormone-binding protein | EC 5.3.4.1 | p55 | PDI | Prolyl 4-hydroxylase subunit beta | P4Hbeta | ERBA2L | CLCRP1 | thyroid hormone-binding protein p55 | Thbp | protocollagen hydroxylase | protein disulfide-isomerase | protein disulfide is
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE)
生物活性
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human P4HB Protein - Enzyme Activity Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions. Materials: Buffer A: 100 mM NaH2PO4, 3 mM DTT, pH 7.0 Buffer B: 100 mM NaH2PO4, 3 mM EDTA, pH 7.0 Recombinant Human P4HB Protein (rp149672) Recombinant Human Insulin Protein (rp218456), 10 mg/mL solution in 0.01 M HCl Procedures： 1. Dilute insulin to 3.5 mg/mL in Buffer B. 2. Incubate diluted insulin for 10 minutes at room temperature (insulin will solubilize) 3. Dilute rhP4HB to 45 µg/mL in Buffer A. 4. Mix 100 µL of 45 µg/mL rhP4HB and 200 µL of 3.5 mg/mL insulin. Create a Substrate Blank with 100 µL Buffer A and 200 µL insulin. 5. Incubate at room temperature for 20 minutes. 6. Load in a clear 96-well plate 100 µL from each reaction vial in duplicate. 7. Read at 650 nm (absorbance) in kinetic mode for 5 minutes. 8. Calculate specific activity: *Adjusted for Substrate Blank **Note: the output of many spectrophotometers in kinetic mode is in mOD. ***Using the pathlength correction of 0.32 cm Per Well: rhP4HB: 0.0015 mg, Insulin: 0.100 mg.
Recombinant Human P4HB Protein (rp149672) - SDS-PAGE 2 μg/lane of Recombinant Human P4HB Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 kDa.
ZJ25F0521434
危险品化学品经营许可证（带存储）