Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of 10-500 ng/mL.
Function Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
规格
产品名称
Recombinant Human RGM-A Protein
别名
重组人RGM-A蛋白 | 重组人反义导向分子A蛋白
英文别名
RGM domain family member A | Repulsive Guidance Molecule A | RGM domain family member A | RGM domain family, member A | RGMA | RGM-A | RGMrepulsive guidance molecule A | RGM
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE)
生物活性
Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent KD <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of 10-500 ng/mL.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human RGM-A Protein (rp150956) - SDS-PAGE 2 μg/lane of Recombinant Human RGM-A Proteinwas resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 33 & 27 kDa under reducing conditions and 53 kDa under non-reducing conditions. Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins. It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (PMID: 14985445,12353034).
ZJ25F0522100
危险品化学品经营许可证（带存储）