Recombinant Human Semaphorin 5A Protein

无动物源

无载体

生物活性

ActiBioPure™

高性能

≥95%(SDS-PAGE)

功能和特点

表达系统: HEK293
Accession #: Q13591
蛋白标签: C-His
生物活性: Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 100-400 ng/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp151382-10μg	10μg	期货
rp151382-50μg	50μg	期货
rp151382-100μg	100μg	期货
rp151382-1mg	1mg	期货

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点击查看蛋白信息: SEMA5A

Accession#: Q13591 (1) Gene ID: 9037 (1) SEMA5A (1) Semaphorin 5A (1)

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基本描述

产品名称	Recombinant Human Semaphorin 5A Protein
别名	重组人Semaphorin 5A蛋白 \| 重组人信号素5A蛋白
英文别名	Sema F \| Semaphorin-F \| FLJ12815 \| sema domain, seven thrombospondin repeats (type 1 and type 1-like) \| Sema F \| SEMA5A \| SEMAF \| Semaphorin 5A \| semaphorin F \| semaphorin-5A \| Semaphorin-F \| semF \| transmembrane domain (TM) and short cytoplasmic domain,
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
生物活性	Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 100-400 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	23-765 aa
序列	EAQGTTQCQRTEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAIAQAFSGPFKYQENSRSAWLPYPNPNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGREALVHIIYLATDYGTIKKVRVPLNQTSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFYRTRSTCIGAQDPYCGWDVVMKKCTSLEESLSMTQWEQSISACPTRNLTVDGHFGVWSPWTPCTHTDGSAVGSCLCRTRSCDSPAPQCGGWQCEGPGMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVGRQRIEMRYCSSDGTSGCSTHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q13591
来源	重组表达
预测分子量	84.4 kDa
SDS-PAGE	95-110 kDa under reducing conditions.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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参考文献

1. Simmons, A D AD, Püschel, A W AW, McPherson, J D JD, Overhauser, J J and Lovett, M M.. (1998) Molecular cloning and mapping of human semaphorin F from the Cri-du-chat candidate interval.. Biochemical and biophysical research communications, (26): [PMID:9464278]
2. Artigiani, Stefania S and 7 more authors.. (2004) Plexin-B3 is a functional receptor for semaphorin 5A.. EMBO reports, [PMID:15218527]
3. Sadanandam, Anguraj A, Rosenbaugh, Erin G EG, Singh, Seema S, Varney, Michelle M and Singh, Rakesh K RK.. (2010) Semaphorin 5A promotes angiogenesis by increasing endothelial cell proliferation, migration, and decreasing apoptosis.. Microvascular research, [PMID:19850054]
4. Li, Xinhua X and Lee, Alan Yiu Wah AY.. (2010) Semaphorin 5A and plexin-B3 inhibit human glioma cell motility through RhoGDIalpha-mediated inactivation of Rac1 GTPase.. The Journal of biological chemistry, (15): [PMID:20696765]
5. Li, X X, Law, J W S JW and Lee, A Y W AY.. (2012) Semaphorin 5A and plexin-B3 regulate human glioma cell motility and morphology through Rac1 and the actin cytoskeleton.. Oncogene, (2): [PMID:21706053]
6. D'Gama, Alissa M AM and 7 more authors.. (2015) Targeted DNA Sequencing from Autism Spectrum Disorder Brains Implicates Multiple Genetic Mechanisms.. Neuron, (2): [PMID:26637798]

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Recombinant Human Semaphorin 5A Protein

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基本描述

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

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