Recombinant Human VCAM1 Protein

无载体

生物活性

ActiBioPure™

无叠氮钠

≥92%(SDS-PAGE)

功能和特点

表达系统: HEK293
Accession #: NP_001069.1/P19320
蛋白标签: N-His
生物活性: Measured by its binding ability in a functional ELISA. Immobilized recombinant human ITGa4 at 2 μg/ml(100 μl/well) can bind Human VCAM-1, the ED50 for this effect is 0.261 µg/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp153094-10μg	10μg	现货
rp153094-50μg	50μg	现货
rp153094-100μg	100μg	现货
rp153094-1mg	1mg	期货

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点击查看蛋白信息: VCAM1

Accession#:NP_001069.1 (1) Accession#:P19320 (4) Gene ID:7412 (4) VCAM1 (5)

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基本描述

产品名称	Recombinant Human VCAM1 Protein
别名	重组人VCAM1蛋白 \| 重组人VCAM1蛋白
英文别名	CD106 \| CD106 antigen \| DKFZp779G2333 \| INCAM-100 \| L1CAM \| MGC99561 \| vascular cell adhesion molecule 1 \| vascular cell adhesion protein 1 \| V-CAM 1 \| VCAM1 \| VCAM-1
规格或纯度	ActiBioPure™, Bioactive, Carrier Free, Azide Free, ≥92%(SDS-PAGE)
产品介绍	Background: VCAM-1, also known as CD106, is an immunoglobulin (Ig)-like adhesion molecule that is mainly expressed in endothelial cells and other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. It plays a critical role in inflammation by recruiting leukocytes to acute and chronic inflammation sites. Alternatively-spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 aa extracellular domain (ECD) that includes seven C2-type immunoglobulin domains, a 22 aa transmembrane segment, and a 19 amino acid (aa) cytoplasmic tail. Within the ECD, human VCAM-1 shares 75% and 76% aa sequence identity with the mouse and rat VCAM-1, respectively. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals, but elevated levels of serum VCAM-1 are indicative of future Atrial Fibrillation incident as well as liver disease. Tumor cells use overexpression of VCAM-1 as means of escaping immune surveillance. Post-translational modifications： Sialoglycoprotein. Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
生物活性	Measured by its binding ability in a functional ELISA. Immobilized recombinant human ITGa4 at 2 μg/ml(100 μl/well) can bind Human VCAM-1, the ED50 for this effect is 0.261 µg/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	Human
氨基酸	109-318 aa
序列	HHHHHHGTDIGSEFQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEIS
纯度	＞92% (SDS-PAGE)
蛋白标签	N-His
蛋白长度	Protein fragment
无载体	Yes
偶联	Unconjugated
Accession #	NP_001069.1/P19320
来源	重组表达
预测分子量	25.0 kDa
SDS-PAGE	30 kDa，under reducing conditions

图片

Protein Bioactivity: Recombinant Human VCAM1 Protein (rp153094)

Recombinant Human VCAM1 Protein (rp153094)-Protein Bioactivity
The binding activity of recombinant human VCAM-1 and recombinant human ITGa4. The ED₅₀ for this effect is <
0.261ug/mL.

SDS-PAGE: Recombinant Human VCAM1 Protein (rp153094)

Recombinant Human VCAM1 Protein (rp153094)-SDS-PAGE
3μg/lane of Recombinant Human VCAM1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 31.9 kDa.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from sterile PBS, pH 7.4, containing 5% Trehalose
复溶	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 2-8℃ 下保存一个月。分装后在 -80℃ 下保存 12 个月。
分子类型	蛋白质

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找到2个结果

批号(Lot Number)	证书类型	日期	货号
ZJ23F0600286	分析证书	23-06-12	rp153094
ZJ23F0600285	分析证书	23-06-12	rp153094

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Recombinant Human VCAM1 Protein

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基本描述

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产品规格参数

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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