Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 10-30 ng/mL. Measured by its ability to bind Recombinant Mouse HGF R/c-MET Fc Chimera in a functional ELISA with an estimated KD <0.2 nM.
Purity ≥95% (SDS-PAGE) Endotoxin level <0.1 EU/μg Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 10-30 ng/mL. Measured by its ability to bind Recombinant Mouse HGF R/c-MET Fc Chimera in a functional ELISA with an estimated KD <0.2 nM.
90.4 & 58.1 & 31.7 kDa, under reducing conditions; 75 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Mouse HGF Protein (rp154071) - SDS-PAGE 3 μg/lane of Recombinant Mouse HGF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 90.4 & 58.1 & 31.7 kDa (α chain 58.1 kDa and β chain 31.7 kDa) under reducing conditions and 75 kDa under non-reducing conditions.