Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Post-translational
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.
70 - 100 kDa, under reducing conditions; 65 - 95 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Mouse ICAM-1/CD54 Protein (rp154082) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5 x 104 cells/well are added to Recombinant Mouse ICAM-1/CD54 Protein (rp154082) coated plates (10 µg/mL with 100 µL/well), approximately 65 - 95% will adhere after 1 hour incubation at 37℃.
Recombinant Mouse ICAM-1/CD54 Protein (rp154082) - SDS-PAGE 3 μg/lane of Recombinant Mouse ICAM-1/CD54 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 70 - 100 kDa under reducing condition and 65 - 95 kDa under non-reducing condition.
ZJ24F1214982
危险品化学品经营许可证（带存储）