IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.
Post-translational modifications:
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
16.1 kDa, under reducing conditions; 16.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
PBS, pH 7.4, 5%Trehalose, 5%Mannitol
复溶
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is recommended to reconstitute to a concentration of 0.1-1mg/mL.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
建议在无菌条件下于 -20℃~ -80℃ 保存 1 年以上。避免反复冻融循环
图片
Recombinant Mouse IL-1 beta Protein (rp154118)-SDS-PAGE 3μg/lane of Recombinant Mouse IL-1 beta was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 16.1 kDa.
Recombinant Mouse IL-1 beta Protein (rp154118) - Protein Bioactivity Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 10 - 50 pg/mL.