Immobilized Recombinant Mouse PD-L1/B7-H1 Protein (rp154493) at 10 μg/mL can bind Recombinant Mouse PD-1 Protein (rp149974), the EC₅₀ of this effect is 3.07 μg/mL.
Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Post-translational: Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8; leading to degradation. Deubiquitinated by USP22; leading to stabilization.
生物活性
Immobilized Recombinant Mouse PD-L1/B7-H1 Protein (rp154493) at 10 μg/mL can bind Recombinant Mouse PD-1 Protein (rp149974), the EC₅₀ of this effect is 3.07 μg/mL.
40 - 54 kDa, under reducing conditions; 38 - 50 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20~-80℃(1年);复溶后建议分装,避免反复冻融。
图片
Recombinant Mouse PD-L1/B7-H1 Protein (rp154493) - Protein Bioactivity Immobilized Recombinant Mouse PD-L1/B7-H1 Protein at 10 μg/mL can bind Recombinant Mouse PD-1 Protein (rp149974), the EC50 of this effect is 3.07 μg/mL.
Recombinant Mouse PD-L1/B7-H1 Protein (rp154493) - SDS-PAGE 3 μg/lane of Recombinant Mouse PD-L1/B7-H1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40 - 54 kDa under reducing condition and 38 -50 kDa under non-reducing condition.