Recombinant Mouse SCF Protein

货号: rp154630
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 无叠氮钠 ? 无叠氮(Azide Free)—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥97%(SDS-PAGE&HPLC)
Accession #
P20826
蛋白标签
No tag
表达系统
E. coli
内毒素水平
<0.1 EU/μg
生物活性
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0 ×10⁵ IU/mg.
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规格
库存
价格
数量
10μg
rp154630-10μg
现货 Stock Image
¥699.90
50μg
rp154630-50μg
现货 Stock Image
¥1,998.90
100μg
rp154630-100μg
现货 Stock Image
¥3,798.90
1mg
rp154630-1mg
期货 Stock Image
¥21,598.90
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为什么选择此级别

ActiBioPure™,PBS Only,无叠氮钠,无载体,生物活性,高性能 级 提供 ≥97%(SDS-PAGE&HPLC) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane It is required for normal hematopoietic function and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant murine SCF is an 18.4kDa polypeptide containing 165 amino acid residues.


Purity

>97% (SDS-PAGE,HPLC)


Function

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.


Post-translational

A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain.

规格

产品名称
Recombinant Mouse SCF Protein
别名
重组小鼠SCF蛋白
英文别名
c-kit Ligand | DCUA | DFNA69 | DKFZp686F2250 | familial progressive hyperpigmentation 2 | FPH2 | FPHH | KIT ligand | Kitl | KITLG | KL-1 | Mast cell growth factor | MGF | MGFSHEP7 | SCF | SCFStem cell factor | SFc-Kit ligand | SHEP7 | SLF | steel factor |
规格或纯度
无载体,生物活性,ActiBioPure™,无叠氮钠,高性能,PBS Only,≥97%(SDS-PAGE&HPLC)
生物活性
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0 ×10⁵ IU/mg.
表达系统
E. coli
种属
小鼠(Mouse)
氨基酸
26-190 aa
序列
MKEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWL RDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENA PKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTL GPEKDSRVSVTKPFMLPPVA
纯度
>97% (SDS-PAGE,HPLC)
蛋白标签
No tag
N-末端序列
Met
无载体
Yes
来源
重组表达
预测分子量
18.4 kDa
SDS-PAGE
16.6 kDa, under reducing conditions; 12.9 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4
复溶
This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be a pportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
如需长期储存,产品应储存在 ≤ -20°C。复溶后请避免反复冻融循环。 在 -20 至 -70°C 下可保存 36 个月;在 2 至 8°C 下可保存 1 个月。
图片

Recombinant Mouse SCF Protein (rp154630) - Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is typically less than 10 ng/mL.

Recombinant Mouse SCF Protein (rp154630)- SDS-PAGE
2.5 μg/lane of Recombinant Mouse SCF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.6 kDa under reducing conditions and 12.9 kDa under non-reducing conditions.

技术文档

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高级数据

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找到6个结果

批号(Lot Number) 证书类型 货号
ZJ23F0800928 分析证书 rp154630
ZJ23F0800929 分析证书 rp154630
ZJ23F0800930 分析证书 rp154630
ZJ25F1129639 分析证书 rp154630
ZJ25F1129638 分析证书 rp154630
ZJ25F1129637 分析证书 rp154630
技术文档和文章
溶液计算器

常见问题

什么是「无载体」?
「无载体」表示该物料经过加工和检测,适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如,电子应用的金属含量、电池应用的粒度、光学应用的折射率,或对水分敏感工艺的水分含量。
什么是「生物活性」?
「生物活性」表示该产品经过加工和检测,适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性,以及在相关情况下的生物活性验证。
本产品应如何储存?
请于?80 °C条件下保存。需超低温保存以维持规定的有效期。
本产品如何运输?
本产品采用干冰配冷袋冷冻运输。收货后请立即拆包,并按上述储存条件保存;处理干冰时请戴隔热手套并保持通风。
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