Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.6-3 µg/mL.
Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
规格
产品名称
Recombinant Mouse Sonic Hedgehog/Shh Protein
别名
重组小鼠Sonic Hedgehog/Shh蛋白 | 重组小鼠音猬因子蛋白 | 重组小鼠 Sonic Hedgehog/Shh 蛋白
英文别名
HHG1 | HHG-1 | HLP3 | HPE3 | MCOPCB5 | sonic hedgehog (Drosophila) homolog | Shh | ShhNC | SMMCI | sonic hedgehog homolog (Drosophila) | sonic hedgehog protein | sonic hedgehog homolog | Sonic Hedgehog | TPT | TPTPS | Hx | Dsh | Hxl3 | M100081 | 9530036O1
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.6-3 µg/mL.
22 kDa, under reducing conditions; 22 & 44 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Mouse Sonic Hedgehog/Shh Protein (rp154674) - SDS-PAGE 3 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 22 kDa under reducing condition and 22 & 44 kDa under non-reducing condition.
ZJ24F0809452
危险品化学品经营许可证（带存储）