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Recombinant Human IL-4 Protein (rp156008) - Protein Bioactivity
Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.
His标签,PBS Only,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining
Description:
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure. Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.
Due to its role in the differentiation of certain immune cell types, IL-4 is commonly used in combination with other growth factors to transform induced pluripotent stem cells into dendritic cells in high numbers. These dendritic cells can then be used for research or clinical applications to improve disease modeling, for screening and cell therapies. Study of IL-4 signaling has led to the development of monoclonal antibodies that can block the signaling pathway at various steps to mitigate the inflammatory response in certain autoimmune diseases. While IL-4 has the capacity to improve immune functions, treatments involving IL-4 have not been utilized due to the dangerous side effects that may result from IL-4 signaling in non-immune cells. Blockade of IL-4 signaling also has been studied as a therapeutic target to suppress inflammation in the tumor microenvironment. Use of IL-4 suppressors can also improve the efficacy of anti-tumor immunotherapies, as blocking IL-4 enhances the activity of tumor-specific T lymphocytes.
Recombinant Human IL-4 Protein (rp156008) - Protein Bioactivity
Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.
Recombinant Human IL-4 Protein (rp156008) - SDS-PAGE
3 μg/lane of Recombinant Human IL-4 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 15.5 kDa under reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp156008 | |
| 分析证书 | rp156008 | |
| 分析证书 | rp156008 | |
| 分析证书 | rp156008 | |
| 分析证书 | rp156008 |
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