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Recombinant Human FGF basic Protein

货号: rp156010
COA
有货
级别和纯度: 无载体 ? 无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 无叠氮钠 ? 无叠氮（Azide Free）—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥95%(SDS-PAGE)
Accession #
P09038
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Measured in a cell proliferation assay using NIH‑3T3 mouse fibroblast cells. The ED50 for this effect is 0.44 ng/mL.
★
规格
库存
价格
数量
10μg
rp156010-10μg
现货 Stock Image
¥459.90
50μg
rp156010-50μg
现货 Stock Image
¥999.90
100μg
rp156010-100μg
现货 Stock Image
¥1,399.90
1mg
rp156010-1mg
期货 Stock Image
¥4,899.90
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Recombinant Human FGF basic Protein
概述 规格 储存与运输 图片 高级数据 质检证书(CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章 溶液计算器

概述


Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules. Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site. The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment. Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases. The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117. There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural. Human 146 aa FGF basic is 97% aa identical to mouse FGF basic.

规格

产品名称
Recombinant Human FGF basic Protein
别名
重组人FGF basic蛋白 | 重组人FGF碱性蛋白
英文别名
Basic fibroblast growth factor | Basic fibroblast growth factor bFGF | BFGF | FGF 2 | FGF B | FGF-2 | Fgf2 | FGF2 basic | FGFB | Fibroblast growth factor 2 (basic) | Fibroblast growth factor 2 | Fibroblast growth factor, basic | HBGF 2 | HBGF-2 | HBGF2 | HBGH 2 | HBGH2 | Heparin binding growth factor 2 precursor | Heparin-binding growth f 展开
规格或纯度
无载体,生物活性,ActiBioPure™,无叠氮钠,高性能,His标签,≥95%(SDS-PAGE)
生物活性
Measured in a cell proliferation assay using NIH‑3T3 mouse fibroblast cells. The ED50 for this effect is 0.44 ng/mL.
表达系统
E. coli
种属
人(Human)
氨基酸
134-288 aa
序列
MGSSHHHHHHSSGLVPRGSHMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
纯度
>95% (SDS-PAGE)
蛋白标签
N-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
19.4 kDa
SDS-PAGE
19.0 kDa, under reducing conditions; 19.0 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM HEPES, 150mM Nacl, 8% Trehalose, pH7.5.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片

Recombinant Human FGF basic Protein (rp156010) - Biological activity

Measured in a cell proliferation assay using NIH‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.44 ng/mL.

Recombinant Human FGF basic Protein (rp156010) - SDS-PAGE
3 μg/lane of Recombinant Human FGF basic Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 19.0 kDa.

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ25F0825500ZJ25F0825500 分析证书 rp156010
ZJ25F0825501ZJ25F0825501 分析证书 rp156010
ZJ25F0825502ZJ25F0825502 分析证书 rp156010
技术文档和文章
成纤维细胞生长因子家族（FGF）
目录： 技术文章
探索主题:
成纤维细胞生长因子家族(FGF) FGF蛋白 FGF抗体 细胞因子和生长因子 FGF抑制剂
The Fibroblast Development Factor (FGF) Family
目录： 技术文章
探索主题:
Fibroblast growth factor family (FGF) FGF protein FGF antibody Cytokines and growth factor FGF inhibitors
溶液计算器
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