Recombinant Human LIF Protein

货号: rp156170

COA

有货

级别和纯度: 无载体 ? 生物活性 ? ActiBioPure™ ? 高性能 ? His标签 ? PBS Only ? ≥95%(SDS-PAGE) 见COA

Accession #

P15018

蛋白标签

N-His

表达系统

E. coli

内毒素水平

<1.0 EU/μg

生物活性

Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.40 ng/mL.

规格

库存

价格

数量

10μg

rp156170-10μg

现货

¥799.90

50μg

rp156170-50μg

现货

¥1,999.90

100μg

rp156170-100μg

现货

¥2,999.90

1mg

rp156170-1mg

期货

¥17,499.90

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规格储存与运输图片技术文档高级数据关联靶点（人）质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无载体,生物活性,高性能级提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称

Recombinant Human LIF Protein

别名

重组人LIF蛋白 | 重组人白血病抑制因子蛋白

英文别名

规格或纯度

无载体, 生物活性, ActiBioPure™, 高性能, His标签, PBS Only, ≥95%(SDS-PAGE), 见COA

生化机理

LIF (leukemia inhibitory factor) is a widely expressed, highly and variably glycosylated, 32‑62 kDa, monomeric, pleiotropic cytokine in the IL‑6 family of helical cytokines. The first exon encoding the signal sequence is alternately spliced, resulting in LIF-D, LIF-M, and LIF‑T mRNAs that produce secreted, extracellular matrix‑associated, and intracellular forms, respectively. LIF-D and LIF-M mRNAs produce identical 180 amino acid (aa) mature sequences. Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. The LIF receptor is a heterodimer of a type I transmembrane ligand‑binding subunit, LIFR (gp190), and the type I transmembrane signal transducing subunit, gp130, signaling especially through STAT3 and JAK kinases. Gp130 and members of the LIFR family also mediate the biological effects of Oncostatin M, Cardiotrophin‑1, Galectin‑10, CNTF, IL‑6, IL‑11, and IL‑27. A soluble LIFR has been reported in the mouse. Depending on the cells and their context, LIF either opposes or favors differentiation. LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells. However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation. LIF is produced by CD4+ T cells in response to activation, and is required by the thymic epithelium to support T cell maturation. LIF expression is up‑regulated by neuronal injury, and promotes motor neuron survival and oligodendrocyte myelination. LIF is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. LIF can function as an autocrine growth factor in some pancreatic cancers, but induces differentiation in the myeloid leukemic cell line M1. Tumor LIF can also induce formation of immunosuppressive tumor‑associated macrophages. LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells. It promotes regulatory T cell and inhibits Th17 cell differentiation, thus promoting tolerance, down‑regulating inflammation, and contributing to immune tolerance during pregnancy and in the nervous system.

生物活性

Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.40 ng/mL.

内毒素水平

<1.0 EU/μg

表达系统

E. coli

种属

人(Human)

氨基酸

23-202 aa

序列

MGSSHHHHHHSSGLVPRGSHMSPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF

蛋白标签

N-His

无载体

Yes

无动物源

Accession #

P15018

来源

重组表达

预测分子量

22.0 kDa

SDS-PAGE

21.5 kDa, under reducing conditions; 20.2 & 37.8 kDa, under non-reducing conditions.

分子类型

蛋白质

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4

浓度

见COA

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

Store at -20°C for 1 year. Avoid freeze/thaw cycle.

图片

Bioactivity: Recombinant Human LIF Protein (rp156170)

Recombinant Human LIF Protein (rp156170) - Bioactivity
Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is typically 0.40 ng/mL.

SDS-PAGE: Recombinant Human LIF Protein (rp156170)

Recombinant Human LIF Protein (rp156170) - SDS-PAGE
3 μg/lane of Recombinant Human LIF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.5 kDa under reducing conditions and 20.2 & 37.8 kDa under non-reducing conditions.
The LIF Protein forms a small amount of dimers in the non-reducing conditions, which may be related to its structure (Uniprot: P15018).

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该级别的完整质量属性和验收标准。

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高级数据

关联靶点（人）

LIF Tbio 白血病抑制因子(Leukemia inhibitory factor) (0 活性数据)

点击查看靶蛋白信息： LIF

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0420652	分析证书	rp156170
ZJ25F0420653	分析证书	rp156170
ZJ25F0420654	分析证书	rp156170

技术文档和文章

神经干细胞移植：神经损伤后的功能重建

目录：技术文章

探索主题:

神经干细胞神经再生髓鞘修复神经祖细胞神经损伤脊髓损伤脑卒中神经回路

Neural Stem Cell Transplantation: Functional Restoration After Neural Injury

目录：技术文章

探索主题:

remyelination neural stem cells neural progenitor cells neural injury spinal cord injury stroke neural regeneration neural circuitry

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分子量

g/mol

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浓度 2 (C2)

体积 2 (V2)

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体积（添加到小瓶中）

质量（小瓶）

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Recombinant Human LIF Protein

为什么选择此级别

储存与运输

质量文档

文献证明

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

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