Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

规格

产品名称

Recombinant Human Nanog Protein

别名

重组人Nanog蛋白 | 重组人类 Nanog 蛋白

英文别名

规格或纯度

无载体,无叠氮钠,His标签,≥95%(SDS-PAGE)

表达系统

E. coli

种属

人(Human)

氨基酸

1-305 aa

序列

MGHHHHHHMSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDVESGGGGSPGRRRRRRRRRRR

纯度

>95% (SDS-PAGE)

蛋白标签

N-His

无载体

Yes

无动物源

来源

重组表达

预测分子量

38.0 kDa

SDS-PAGE

45.2 kDa, reducing conditions; 38.6, 41.1 and 78.7 kDa, non-reducing condition

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, 8M Urea, PH 7.4

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。

图片

SDS-PAGE: Recombinant Human Nanog Protein (rp156660)

Recombinant Human Nanog Protein (rp156660) - SDS-PAGE
3 μg/lane of Recombinant Human Nanog Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 45.2 kDa under reducing conditions and 38.6 & 41.1 & 78.7 kDa under non-reducing conditions.