Measured by its binding ability in a functional ELISA. When Recombinant Human R Spondin 3 is coated at 0.25 μg/mL, Recombinant Human ZNRF3 binds with an apparent Kd <3 nM.
Function E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby restricting the size of the intestinal stem cell zone. Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification.
Zinc and Ring Finger 3 | BK747E2.3 | KIAA1133 | novel C3HC4 type Zinc finger (ring finger) | RNF203 | zinc and ring finger 3 | zinc/RING finger protein 3 | ZNRF3 | Zinc and Ring Finger 3 | BK747E2.3 | KIAA1133 | novel C3HC4 type Zinc finger (ring finger)
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE)
生物活性
Measured by its binding ability in a functional ELISA. When Recombinant Human R Spondin 3 is coated at 0.25 μg/mL, Recombinant Human ZNRF3 binds with an apparent Kd <3 nM.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human ZNRF3 Protein (rp166398) - SDS-PAGE 2 μg/lane of Recombinant Human ZNRF3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 18 - 20 kDa. The expression profile and molecular weight observed may be due to post-translational modifications.
ZJ25F0622450
危险品化学品经营许可证（带存储）