级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥95%(SDS-PAGE)
Accession #
Q9UP38
蛋白标签
C-hFc & His
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-1/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL. Optimal concentrations should be determined by each laboratory for each application.
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B.
Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-1/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL. Optimal concentrations should be determined by each laboratory for each application.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human Frizzled-1 Protein (rp166452) - SDS-PAGE 2 μg/lane of Recombinant Human Frizzled-1 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing the band at 54 - 68 kDa
ZJ25F0420649
危险品化学品经营许可证（带存储）