Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. Recombinant Human Hip inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity in a C3H10T1/2 mouse embryonic fibroblast cell alkaline phosphatase induction assay with an ED50 = 0.1-0.6 µg/mL.
Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. Recombinant Human Hip inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity in a C3H10T1/2 mouse embryonic fibroblast cell alkaline phosphatase induction assay with an ED50 = 0.1-0.6 µg/mL.
63 - 81 kDa, under reducing conditions; 116 - 162 & 350 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
图片
Recombinant Human Hip Protein (rp167147) - SDS-PAGE 3 μg/lane of Recombinant Human Hip Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 63 - 81 kDa under reducing condition and 116 - 162 & 350 kDa under non-reducing condition.