His标签,PBS Only,无动物源,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-3 is alternatively known as myeloid cell surface antigen CD33 and GP67. Human Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. Siglec-3 expression is restricted to cells of myelomonocytic lineage. It binds sialic acid preferring alpha 2,3-linkage over alpha 2,6-linkage. Studies indicated that Siglec-3 recruits SHP-1 and SHP-2 to its ITIMs. When co-cross-linking with Fc gamma R1, Siglec3- inhibits tyrosine phosphorylation and calcium mobilization, suggesting Siglec-3 can mediate inhibitory signals.
Recombinant Human Siglec-3/CD33 Protein (rp169578) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is < 6 µg/mL.
Recombinant Human Siglec-3/CD33 Protein (rp169578) - ELISA
Immobilized Recombinant Human Siglec-3/CD33 Protein (rp169578) at 1.0 μg/mL can bind Gemtuzumab (anti-CD33) (Ab175859) with the EC
₅₀
of 36.93 ng/mL.
Recombinant Human Siglec-3/CD33 Protein (rp169578) - ELISA
Immobilized Recombinant Human Siglec-3/CD33 Protein (rp169578) at 1.0 μg/mL can bind Vadastuximab (anti-CD33) (Ab182778) with the EC
₅₀
of 10.59 ng/mL.
Recombinant Human Siglec-3/CD33 Protein (rp169578) - SDS-PAGE
3 μg/lane of Recombinant Human Siglec-3/CD33 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.5-55.1 kDa.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0606548 |
分析证书 | rp169578 |
| ZJ24F0606549 |
分析证书 | rp169578 |
| ZJ24F0606550 |
分析证书 | rp169578 |
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