CD63, also known as Tspan-30, is a member of the tetraspanin family of proteins that mediate signal transduction events which play a role in the regulation of cell development, activation, growth and motility. These membrane proteins are characterized by the presence of four transmembrane domains, a small extracellular loop (SEL) and a large extracellular loop (LEL). Regions of the tetraspanin LEL are known to associate with integrins to regulate membrane protein trafficking, leukocyte recruitment, and adhesion processes. Deficiency of CD63 is associated with Hermansky-Pudlak syndrome. Human CD63-LEL shares 67% and 66% aa sequence identity with mouse and rat CD63-LEL, respectively. CD63 has been identified as a cell surface binding partner for tissue inhibitor of metalloproteinase 1 (TIMP-1), modulating the tetraspanin/integrin signaling complex which regulates cell survival and polarization.
Post-translational: Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
生物活性
Immobilized Recombinant Human CD63 Protein (rp169586) at 1.0 μg/mL can bind Recombinant CD63 Antibody (Ab095139) with the EC50 of 7.9 ng/mL.
14.8-31.8 kDa, under reducing conditions; 15.6-32.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human CD63 Protein (rp169586) - ELISA Immobilized Recombinant Human CD63 Protein (rp169586) at 1.0 μg/mL can bind Recombinant CD63 Antibody (Ab095139) with the EC50 of 7.9 ng/mL.
Recombinant Human CD63 Protein (rp169586) - SDS-PAGE 3 μg/lane of Recombinant Human CD63 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.8-31.8 kDa under reducing conditions and 15.6-32.1 kDa under non-reducing conditions.