Protein Function： Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
规格
产品名称
Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein
别名
重组人 Pro-Collagen I alpha 1/COL1A1蛋白 | 重组人I型胶原前体蛋白1 | 重组人类前胶原蛋白 I alpha 1/COL1A1 蛋白
英文别名
COL1A1 | Alpha-1 type I collagen | COL-IA1 | Collagen 1 alpha 1 | Collagen 1 | collagen alpha 1 chain type I | collagen alpha-1(I) chain | Collagen I alpha 1 | collagen of skin, tendon and bone, alpha-1 chain | Collagen Type I Alpha 1 Chain | collagen, ty
规格或纯度
无动物源,无载体,生物活性,His标签,≥90%(SDS-PAGE)
生物活性
Immobilized Recombinant Human COL1A1 Protein (rp169600) at 1.0 μg/mL can bind Collagen I Antibody (Ab097170) with the EC50 of 29.15 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein (rp169600) - ELISA Immobilized Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein (rp169600) at 1.0 μg/mL can bind Collagen I Antibody (Ab097170) with the EC₅₀ of 29.15 ng/mL.
Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein (rp169600) - SDS-PAGE 3 μg/lane of Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 77.9 kDa under reducing conditions and above 250.0 kDa under non-reducing conditions. Non-reducing (NR) conditions suggests that a portion of the protein forms tetramer. This may be mediated by (or stabilized by) disulfide bonds (Uniprot: P02452).
ZJ25F0825299
危险品化学品经营许可证（带存储）