Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed:12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed:30279168).
生物活性
Fully biologically active determined by the dose dependent proliferation of TF-1 cells. The ED50 for this effect is 13.28 ng/ml.
21.1-24.2 kDa, under reducing conditions; 21.1-24.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 8% Trehalose, 5% Mannitol,pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human IL-11 Protein (rp169619) - Protein Bioactivity Fully biologically active determined by the dose dependent proliferation of TF-1 cells. The ED₅₀ for this effect is 13.28 ng/ml.
Recombinant Human IL-11 Protein (rp169619) - SDS-PAGE 3 μg/lane of Recombinant Human IL-11 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.1-24.2 kDa under reducing conditions and 21.1-24.2 kDa under non-reducing conditions.