计算溶液所需的质量、体积或浓度。
Recombinant Human 4-1BB/TNFRSF9 Protein (rp169624) - ELISA
Measured by its binding ability in a functional ELISA. Human 4-1BB/TNFRSF9 (rp169624) at 1.0 μg/mL can bind Urelumab (anti-TNFRSF9) (Ab170654) with the EC₅₀ of 47.08 ng/mL.
ActiBioPure™,His标签,PBS Only,无动物源,无叠氮钠,无载体,生物活性 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description:
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.
Recombinant Human 4-1BB/TNFRSF9 Protein (rp169624) - ELISA
Measured by its binding ability in a functional ELISA. Human 4-1BB/TNFRSF9 (rp169624) at 1.0 μg/mL can bind Urelumab (anti-TNFRSF9) (Ab170654) with the EC₅₀ of 47.08 ng/mL.
Recombinant Human 4-1BB/TNFRSF9 Protein (rp169624) - SDS-PAGE
3 μg/lane of Recombinant Human 4-1BB/TNFRSF9 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.8-38.6 kDa under reducing conditions and 28.4-37.9 kDa under non-reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp169624 | |
| 分析证书 | rp169624 | |
| 分析证书 | rp169624 | |
| 分析证书 | rp169624 |
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